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Readable research linked to original sources
Browse normalized, publication-ready research with direct links to its evidence and source.
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Readable research linked to original sources
Browse normalized, publication-ready research with direct links to its evidence and source.
21 articles
Newest firstWe profiled antibiotic resistance genes, bacterial communities, and mobile genetic elements in untreated hospital wastewater from three tertiary hospitals in Metro Manila using shotgun metagenomic sequencing. The resistome analysis revealed high abundances of genes known to confer resistance against sulfonamides (sul1, sul2), aminoglycosides (aadS), and macrolides/streptogramins (msrE, mphE). High-risk resistance…
Open article record in new tab ↗BackgroundAntibiotic resistance is a global priority in healthcare. Leveraging thousands of whole-genome sequences, here we reveal the core resistance determinants of oral streptococci, focusing on assessing pattern variability and gene exchange across commensals and pathogens. MethodsGenomic information was obtained from the National Center for Biotechnology Information. Determinants of antibiotic resistance were…
Sow endometritis is usually caused by multiple species of pathogenic bacteria. Numerous isolates from endometritis patients have developed antimicrobial resistance. Thus, novel antibacterial agents and strategies to combat endometritis are needed. A total of 526 bacteria, including Staphylococcus spp. (26.3%), Streptococcus spp. (12.3%), E. coli (28.9%), Enterococcus spp. (20.1%), Proteus spp. (9.5%), and Coryneba…
The emergence of antibiotic-resistant bacteria has attracted interest in the field of endolysins. Here, we analyzed the diversity of Streptococcus endolysins and identified a new endolysin, Ply2741, that exhibited broad-spectrum bactericidal activity. Our results demonstrated that Ply2741 could effectively eradicate multidrug-resistant gram-positive pathogens in vitro and in vivo. Structural analysis revealed that…
Open article record in new tab ↗Most equipment used in aquaculture farms is made of plastic. Plastics-associated biofilms may contain potential human pathogenic bacteria (PHPB) and antibiotic-resistant bacteria (ARB). Understanding the influence of farming practices on the biofouling development and composition is thus essential to control associated microbiological risks. We combined results from metabarcoding analyses, bacterial cultures, and…
IntroductionSelf-medication, while convenient for managing minor illnesses, poses significant public health risks when practiced without adequate knowledge or oversight. In Bangladesh, especially in urban areas, self-medication is widespread due to limited healthcare access and weak pharmaceutical regulation. This study aims to assess the knowledge, attitudes, and practices of self-medication among urban adult res…
Open article record in new tab ↗This cross-sectional study aimed to assess the frequency of Enterobacterales producing extended-spectrum {beta}-lactamases (ESBL) and to conduct a molecular characterisation of the ESBL resistance genes circulating in this region of Burkina Faso. Enterobacterales were isolated from clinical samples collected from the two main healthcare centres in Dedougou (Regional Hospital Center and Notre Dame de Bonne Delivran…
Multidrug-resistant Gram-positive bacteria, including bacteria from the genus Staphylococcus, are currently a challenge for medicine. Therefore, the development of new antimicrobials is required. Promising candidates for new antistaphylococcal drugs are phage endolysins, including endolysins from thermophilic phages against other Gram-positive bacteria. In this study, the recombinant endolysin LysAP45 from the the…
BackgroundEscherichia coli and Klebsiella pneumoniae rank among the primary bacterial culprits in neonatal infections and fatalities in sub-Saharan Africa. This study sought to characterize the phenotypic and genotypic features of Escherichia coli and Klebsiella pneumoniae in a labour ward in Yaounde, Cameroon. MethodsA prospective and cross-sectional study spanning five months, from February 21 to June 30, 2022.…
Bacteriophage endolysins are bacteriolytic enzymes that have been explored as potential weapons to fight antibiotic-resistant bacteria. Despite several studies support the application of endolysins as enzybiotics, detailed knowledge on cellular and enzymatic factors affecting their lytic activity is still missing. The bacterial membrane proton motive force (PMF) and certain cell wall glycopolymers of Gram-positive…
Staphylococcus aureus often leads to severe skin infections. However, S. aureus is facing a crisis of antibiotic resistance. The combination of phage and antibiotics is effective for drug-resistant S. aureus infections. Therefore, it is worth exploiting novel antibacterial agents to cooperate with antibiotics against S. aureus infections. Herein, a novel chimeric lysin ClyQ was constructed, which was composed of a…
Acinetobacter baumannii is a Gram-negative healthcare-associated pathogen that poses a major health concern due to increasing multidrug resistance. The Gram-negative cell envelope is a key barrier to antimicrobial entry and includes an inner and outer membrane. The outer membrane has an asymmetric composition that is important for structural integrity and barrier to the environment. Therefore, Gram-negative bacter…
The goal of this study was to determine minimum selection concentrations of various antibiotics using four manure-originated multi-drug resistant plasmids in a surrogate Escherichia coli host. These plasmids carried genes conferring resistance phenotypes to several antibiotic classes including {beta}-lactams, lincosamides, phenicols, macrolides, sulfonamides and tetracyclines. The minimum selection concentrations…
Open article record in new tab ↗Cannabinoid receptor 2 (CB2) is of interest as a much-needed target for the treatment or prevention of several neurogenerative diseases. However, CB2 agonists, particularly phytocannabinoids, have been ascribed antimicrobial properties and are associated with the induction of microbiome compositional fluxes. When developing novel CB2 therapeutics, CB2 engagement and antimicrobial functions should both be considere…
Open article record in new tab ↗Plasmids are infectious double stranded DNA molecules that are found within bacteria. Horizontal gene transfer promotes successful spread of different types of plasmids within or among bacteria species, making their detection an important task for guiding clinical treatment. We used whole genome sequenced data to determine the prevalence of plasmid replicons in clinical bacterial isolates, the presence of resistan…
BackgroundIndias typhoid burden estimates are based on a limited number of population-based studies and data from a grossly incomplete disease surveillance system. In this study, we estimated the total and sex- and age-specific antibiotic prescription rates for typhoid. MethodsWe used systematic antibiotic prescription by private sector primary care physicians in India. We categorized antibiotics using the WHO cla…
Open article record in new tab ↗There is an urgent need to improve our understanding of how new antibiotic resistant organisms emerge and spread. A high-priority resistance threat is the ST258 lineage of carbapenem-resistant Klebsiella pneumoniae. Here, we studied resistance to the last-line drug colistin among ST258 by tracking its evolution across 21 U.S. hospitals over the course of a year. Phylogenetic analysis supported a significant fitnes…
1BackgroundAntimicrobial resistance is a significant global threat, posing major public health risks and economic costs to healthcare systems. Bacterial cultures are typically used to diagnose healthcare-acquired infections (HAI); however, culture-dependent methods provide limited presence/absence information and are not applicable to all pathogens. Next generation sequencing (NGS) has the capacity to detect a wid…
With an estimated 440,000 active cases occurring each year, medical device associated infections pose a significant burden on the US healthcare system, costing about $9.8 billion in 2013. Staphylococcus epidermidis is the most common cause of these device-associated infections, which typically involve isolates that are multi-drug resistant and possess multiple virulence factors. S. epidermidis is also frequently a…
Open article record in new tab ↗The Atacama Desert, the driest and oldest desert in the world, is a hostile environment for life. Despite the inhospitable conditions, bacterial sequences detected in this location suggest rich bacterial life. This study tested the idea that certain bacteria would thrive in this location and that some of them could be cultivated permitting further characterization. Environmental surface soil samples from 1-5 cm de…