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Research linked to this compound
119 rights-cleared articles, ordered newest first.
The objective of this study was to explore non-aqueous spray-freeze-drying (SFD) as a formulation strategy to stabilize and improve the dissolution of Cannabis sativa full extracts. The medicinal use of Cannabis sativa has strongly intensified over the last decade. Here, Cannabis flos full extracts prepared by tert-butanolic solutions with 25% solid content were directly processed via spray-freeze-drying (SFD) wit…
Cannabinoid content can vary several-fold across Cannabis sativa L. cultivars that carry functional, closely related CBDA synthase (CBDAS) and THCA synthase (THCAS) alleles, a difference that coding-sequence variation among functional alleles does not fully explain. Structural and copy-number variation at the synthase loci, the functional or pseudogenised state of synthase alleles, and linkage at the B locus accou…
Accurate quantification of Δ9-tetrahydrocannabinol (THC) and Δ9-tetrahydrocannabinolic acid (THCA) across diverse cannabis- and hemp-derived products remains challenging due to severe matrix effects, wide concentration variability, and the need for matrix-matched calibration in traditional LC-MS workflows. Here, we develop an in-sample calibration curve (ISCC) method based on multiple isotopologue reaction monitor…
Cannabis sativa yields a wide range of bioactive compounds, including terpenes, flavonoids, and cannabinoids. Tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), and cannabichromenic acid (CBCA) are the acidic biosynthetic precursors of the neutral cannabinoids Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD), which have been the subject of much research. This review exami…
The enzymes Cannabichromenic Acid Synthase (CBCAS), Cannabidiolic Acid Synthase (CBDAS) and Tetrahydrocannabinolic Acid Synthase (THCAS) are together the major cannabinoid synthase enzymes responsible for the biosynthesis of their respective metabolites from a common precursor Cannabigerolic Acid (CBGA). As the catalysts responsible for generating biological molecules of significant pharmaceutical value, there has…
Recently developed dried blood analysis methods for cannabinoid quantitation utilize small blood volumes, making them microsampling-compatible, but are limited by hematocrit-related bias for dried blood spots (DBSs) and higher consumable costs for volumetric absorptive microsampling (VAMS®). To address these issues, we developed a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method ca…
IntroductionPeroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-activated nuclear receptor with broad therapeutic relevance across various pathologies, including type 2 diabetes, obesity, cancer, and inflammatory disorders. Cannabinoids are a class of terpene-phenolic compounds from Cannabis sativa L. that have been shown to act as partial agonists of PPARγ. Among them, the acidic forms Δ9-tetrahy…
Cannabinoids, such as tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA) and cannabichromenic acid (CBCA), are bioactive and medicinally relevant compounds found in the cannabis plant (Cannabis sativa L.). These three compounds are synthesised from a single precursor, cannabigerolic acid (CBGA), through regioselective reactions catalysed by different cannabinoid oxidocyclase enzymes. Despite the importa…
Introduction The study of hemp-based cannabinoids has focused primarily on cannabidiol (CBD). Cannabis sativa however, naturally synthesizes cannabidiolic acid (CBDA) as opposed to the better-known neutral derivative. The precursor to cannabidiolic CBDA is cannabigerolic acid (CBGA) and cultivar extracts that contain both acidic cannabinoids are available as animal supplements. Pharmacokinetically these acidic can…
Chronic osteoarthritis (COA) is a progressive and degenerative condition that causes joint inflammation and pain, often requiring long-term pharmacological management. Conventional treatments may lead to adverse effects, tolerance, and limited analgesic efficacy. This randomized, double-blind clinical trial evaluated the analgesic potential of a full-spectrum Cannabis sativa oil extract administered orally twice d…
Plant growth-promoting rhizobacteria (PGPR) establish beneficial associations with plants, enhancing nutrient uptake, growth, and stress tolerance. Cannabis sativa L., a medicinal plant producing over 300 specialized metabolites with relevant medicinal properties, remains underexplored for PGPR influence on its metabolism. This study assessed the ability of four PGPR taxa: Bacillus, Pseudomonas, Flavobacterium, an…
Cannabinoids comprise a diverse class of bioactive natural products with important therapeutic potential, but efficient microbial production remains limited by pathway bottlenecks and challenges in engineering key biosynthetic enzymes. Here, we develop a machine learning-guided approach to engineer olivetolic acid cyclase (OAC), a critical control point in cannabinoid biosynthesis that governs both pathway flux an…
Cannabidiol (CBD), a non-psychotropic cannabinoid derived from Cannabis plants, is increasingly explored for its potential therapeutic applications in veterinary medicine. This study aimed to evaluate the impact of CBD oil on the minimum alveolar concentration of isoflurane (MACiso) in cats. Sixteen healthy cats underwent isoflurane anesthesia, and the MACiso was determined using the tail-clamping technique both a…
Introduction Research on optimizing light intensity to maximize phytochemical production during hemp flowering is limited, despite growing global demand. We investigated the effects of light-emitting diode (LED) intensity on hemp growth, cannabinoid content, and gene expression. Methods Hemp plants (Cannabis sativa 'Queen Dream') were grown under white LEDs at light intensities of 200, 400, and 600 μmol·m-2·s-1 wi…
Cannabis sativa L. is divided into three main groups: drug-type, intermediate-type, and fiber-type. The presence of tetrahydrocannabinol (THC) exceeding 0.2-0.3% in drug-type and intermediate Cannabis that utilized for recreational and medicinal purposes renders them illegal due to potential mental health implications. Fiber-type contains high cannabidiol (CBD) and low THC, making it suitable for household use suc…
The incorporation of Cannabis sativa L. seeds into barley wort was investigated to enhance the functional profile of beer. Hemp seeds (cv. Henola) were malted via controlled steeping, germination, and kilning, then added to barley malt at 10% and 30% (w/w) in both malted and unmalted forms. Standard congress mashing produced worts whose physicochemical parameters (pH, extract, colour, turbidity, filtration and sac…
Cannabis compounds are well-known for their therapeutic applications in the treatment of various health issues. These substances, mainly cannabinoids, are known for their antimicrobial properties and ability to interact with various cells through endocannabinoid receptors. However, the limitations of cannabis extract, particularly its viscosity, stickiness, and low bioavailability when applied topically, limit its…
Background/Objectives: According to the World Drug Report 2025, cannabis is the most abused drug in the world, being sold in illicit markets in various physical forms ranging from herbal cannabis to cannabis resin and liquid cannabis. Currently, the methods used for cannabis identification are largely based on the morphological features and chemical content of the product. In this respect, identification could be…
Cannabis sativa L. is an ancient species that has been cultivated over the years for various applications, including recreational and medicinal use. Nowadays, the demand for its products has grown significantly, leading to an increase in the search for medicinal oils and a rise in smuggling, making it the main trafficked drug. In this context, our work optimized the extraction process of six cannabinoids in Cannab…
Cannabinoid molecules are the family of molecules that bind to the cannabinoid receptors (CB1 and CB2) of the human body and cause changes in numerous biological functions including motor coordination, emotion, and pain reception. Cannabinoids occur either naturally in the Cannabis Sativa plant or can be produced synthetically in the laboratory. The need for accurate analytical methods for analyzing cannabinoid mo…