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Readable research linked to original sources
Browse normalized, publication-ready research with direct links to its evidence and source.
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Readable research linked to original sources
Browse normalized, publication-ready research with direct links to its evidence and source.
25 articles
Newest firstThe influence of Thermomyces lanuginosus on the chemical structure of biopolymers and lipids from hemp pomace during solid-state fermentation (SSF) was studied using Nuclear Magnetic Resonance (NMR) spectroscopy. The samples of hemp pomace before, during and after SSF were used for the isolation of biopolymers (lignin, cellulose, hemicellulose) and lipids, which were subsequently analyzed using solution and solid-…
Open article record in new tab ↗BACKGROUND: Hemp (Cannabis sativa L.) is one of the oldest cultivated plants in human history. It is a valuable plant that has economic value for all industrial branches. The hemp plant has fibers of the highest quality. Accordingly, research aiming to enhance the fiber yield of hemp has significant agronomic and industrial relevance. METHODS: To this end, three soilless media treatments were applied to the modifi…
Open article record in new tab ↗The study explores the potential of thermoplastic biocomposites made from cellulose acetate, modified hemp seed hull particulate fillers and environmentally friendly plasticizer triacetin. Emphasizing the environmental advantages of utilizing natural materials, the research demonstrates the impact of different hemp hull chemical modification, such as alkali treatment or acetylation, on the mechanical properties of…
Filamentous fungi use various enzymes to degrade cellulose, some of which contain cellulose-binding domains (CBDs), most of which belong to carbohydrate-binding module family 1 (CBM1). We recently identified the novel fungal CBD, CBM104, from Gloeophyllum trabeum. Reportedly, CBM104 specifically binds to native crystalline cellulose, not to amorphous or artificially modified crystalline cellulose, exhibiting a uni…
Open article record in new tab ↗Endochitinase Chit33 has shown great potential in converting chitin, a recalcitrant waste, into bioactive chitooligosaccharides (COS). This study evaluates how cellulose-binding domain (CBD) and chitin-binding domain (ChBD) affect the hydrolytic activity and product specificity of Chit33. Recombinant proteins were produced and isolated with a simple yeast extracellular medium concentration. The domain functionalit…
Background One of the leading current trends in technology is the miniaturization of devices to the microscale and nanoscale. The highly advanced approaches are based on biological systems, subjected to bioengineering using chemical, enzymatic and recombinant methods. Here we have utilised the biological affinity towards cellulose of the cellulose binding domain (CBD) fused with recombinant proteins. Results Here…
The increasing antibiotic resistance among bacteria challenges the biotech industry to search for new antibacterial molecules. Endolysin TP84_28 is a thermostable, lytic enzyme, encoded by the bacteriophage (phage) TP-84, and it effectively digests host bacteria cell wall. Biofilms, together with antibiotic resistance, are major problems in clinical medicine and industry. The challenge is to keep antibacterial mol…
Open article record in new tab ↗Proper retting process of hemp stems, in which efficient separation of cellulose fiber from the rest of the stem is promoted by indigenous microorganisms able to degrade pectin, is essential for fiber production and quality. This research aimed to investigate the effect of a pre-treatment dew retting in field of hemp stalks on the pectinolytic enzymatic activity and microbiota dynamic during lab-scale water rettin…
Open article record in new tab ↗Both annual (cotton, flax, hemp, etc.) and perennial (trees and grasses) plants can serve as a source of cellulose for fiber production. In recent years, the perennial herbaceous plant miscanthus has attracted particular interest as a popular industrial plant with enormous potential. This industrial crop, which contains up to 57% cellulose, serves as a raw material in the chemical and biotechnology sectors. This s…
Cannabidiol (CBD) is a natural terpenophenolic compound with known pharmacological activities, but the poor solubility of CBD in water limits its widespread use in medicine and pharmacy. Polymeric (nano)carriers demonstrated high potential for enhancing the solubility and therapeutic activity of lipophilic drugs such as CBD. Here, we report the elaboration of a novel hydroxypropyl cellulose (HPC)-based in situ gel…
Background Mycobacterium tuberculosis (Mtb) forms physiologically relevant biofilms harboring drug-tolerant bacteria. This observation has brought the study of mycobacterial biofilms to the forefront of tuberculosis research. We established earlier that dithiothreitol (DTT) mediated reductive stress induces cellulose-rich biofilm formation in Mtb cultures. The molecular events associated with the DTT-induced biofi…
Open article record in new tab ↗Paper substrates are promising for development of cost-effective and efficient point-of-care biosensors, essential for public healthcare and environmental diagnostics in emergency situations. Most paper-based biosensors rely on the natural capillarity of paper to perform qualitative or semi-quantitative colorimetric detections. To achieve quantification and better sensitivity, technologies combining paper-based su…
Cellobiohydrolases directly convert crystalline cellulose into cellobiose and are of biotechnological interest to achieve efficient biomass utilization. As a result, much research in the field has focused on identifying cellobiohydrolases that are very fast. Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalyti…
Automotive and industrial design companies have profusely used commodity materials like glass fiber-reinforced polypropylene. These materials show advantageous ratios between cost and mechanical properties, but poor environmental yields. Natural fibers have been tested as replacements of glass fibers, obtaining noticeable tensile strengths, but being unable to reach the strength of glass fiber-reinforced composite…
Open article record in new tab ↗Background Several types of phospholipases have been described in phospholipids modification. The majority of phospholipase D (PLD) superfamily members can catalyze two separate reactions: the hydrolysis of phospholipids to produce phosphatidic acid (PA) and the transphosphatidylation of phosphatidyl groups into various phosphatidyl alcohols to produce modified phospholipids. Transphosphatidylation is a useful bio…
Cellulose-binding domains (CBDs) are protein domains with cellulose-binding activity, and some act as leaders in the localization of cellulosomal scaffoldin proteins to the hydrophobic surface of crystalline cellulose. In this study, we found that a CBD fusion enhanced and improved soluble β-glucuronidase (GusA) enzyme properties through the formation of an artificially oligomeric state. First, a soluble CBD fused…
Open article record in new tab ↗The cellulose binding domain (CBD) of cellulase binding to cellulosic materials is the initiation of a synergistic action on the enzymatic hydrolysis of the most abundant renewable biomass resources in nature. The binding of the CBD domain to cellulosic substrates generally relies on the interaction between the aromatic amino acids structurally located on the flat face of the CBD domain and the glucose rings of ce…
Open article record in new tab ↗Cannabis sativa L. is an important herbaceous species originating from Central Asia, which has been used in folk medicine and as a source of textile fiber since the dawn of times. This fast-growing plant has recently seen a resurgence of interest because of its multi-purpose applications: it is indeed a treasure trove of phytochemicals and a rich source of both cellulosic and woody fibers. Equally highly intereste…
Open article record in new tab ↗In this study a largely available lignocellulose feedstock hemp (Cannabis sativa), obtained as an industrial waste, was used for cellulose extraction. The extraction of cellulose microfibres from hemp biomass was conducted by alkaline treatment and an acidification process. The extracted cellulose microfibres were characterised using Fourier-transformed infrared spectroscopy (FTIR), Scanning electron microscopy (S…
Open article record in new tab ↗The fabrication of cellulose-spider silk bio-nanocomposites comprised of cellulose nanocrystals (CNCs) and recombinant spider silk protein fused to a cellulose binding domain (CBD) is described. Silk-CBD successfully binds cellulose, and unlike recombinant silk alone, silk-CBD self-assembles into microfibrils even in the absence of CNCs. Silk-CBD-CNC composite sponges and films show changes in internal structure a…