Integrated Metabolomic and Proteomic Analyses Reveal Differential Regulation of the Citrate Cycle and Pentose Phosphate Pathway by Cannabis sativa Extract and the Synthetic Cannabinoid HU210 in GT1-7 Neuronal Cells: Sequential Extraction of Metabolites and Proteins from a Single Cell Pellet

Yujin Natori, Dai Mizuno, Masaru Doshi, Masahiro Kawahara, Akira Ishii

Highlights What are the main findings? HU210 altered both the metabolome and proteome, whereas Cannabis extract altered the metabolome with minimal proteome change in GT1-7 cells.Five pathways—citrate cycle, pentose phosphate pathway, pyruvate, butanoate, and glycerolipid metabolism—were differentially regulated by HU210 and Cannabis extract. What are the implications of the findings? Distinct metabolome–proteome…