First report of Meloidogyne hapla on hemp (Cannabis sativa) in Oregon

Article type: Research Article

Keywords: detection, hemp, identification, northern root-knot nematode, PCR-RFLP, sequencing, specific-primers

Authors: Lester Núñez-Rodríguez, Hannah Rivedal, Amy Peetz, Cynthia M. Ocamb, Inga Zasada

Affiliations: Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331; USDA-ARS Forage Seed and Cereal Research Unit, Corvallis, OR 97331; USDA-ARS Horticultural Crops Disease and Pest Management Research Unit, Corvallis, OR 97331

License: © 2024 Lester Núñez-Rodríguez et al., published by Sciendo CC BY 4.0 This work is licensed under the Creative Commons Attribution 4.0 International License.

Article links: DOI: 10.2478/jofnem-2024-0008  |  PubMed: 38495931  |  PMC: PMC10940274

Relevance: Core topic: mentioned in title and keywords

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The Pacific Northwest (PNW) of the United States (Idaho, Oregon, and Washington) encompasses a wide range of ecoclimates, which allows for the production of a wide diversity of crops (ref. USDA-NASS, 2019). A crop that has recently gained importance in Washington and Oregon is hemp (Cannabis sativa). In 2021, total hemp production in these states generated more than $249 million (ref. USDA-NASS, 2022). However, as with many other crops, hemp production faces challenges due to pests and diseases, including plant-parasitic nematodes. Endoparasitic nematodes top the list of the most important plant-parasitic nematodes and include Meloidogyne, arguably the most important plant-parasitic nematode globally (ref. Jones et al., 2013). This nematode can parasitize more than 2,000 plant species, including non-agricultural and economically important agricultural crops (ref. Agrios, 2005; ref. Núñez-Rodríguez et al., 2022). Despite the growing importance of hemp in the PNW, there is little information about plant-parasitic nematodes associated with the crop in this region (ref. Núñez-Rodríguez et al., 2023).

In 2022, during a survey of plant-parasitic nematodes in the PNW, Meloidogyne sp. was found in a composite root sample of hemp ‘Suver Haze’ (21,336 second-stage juveniles/100 g of wet root tissue) from a field located in Benton County, Oregon. No galls were observed on the hemp root sample. Meloidogyne sp. was extracted from roots under intermittent mist (ref. Zasada et al., 2015). Second-stage juveniles (J2) (n = 15) were hand-picked for morphological characterization. Nematodes were temporarily mounted, photographed, and measured using an Olympus BX51 microscope (Melville, NY) with an Olympus DP72 camera (Center Valley, PA). The following measurements (mean μm ± standard deviation) were determined: body length was 429.6 ± 16.5 (Fig. 1A), body width 14.6 ± 0.4, stylet was weak with a length of 13.9 ± 0.3 and knobs were rounded (Fig. 1B), anal body diameter was 10.7 ± 0.4, tail with length was 61 ± 2.5 with different shapes (Figs. 1C,D), and hyaline region length was 14.2 ± 1.4. Measurements of the Meloidogyne sp. population collected from Oregon hemp roots were within the reported range for Meloidogyne hapla (ref. Hunt and Handoo, 2009; ref. Meressa et al., 2015; ref. Sohrabi et al., 2015; ref. Akyazi et al., 2017).

To confirm the identification of M. hapla, DNA was extracted from single J2 (n = 8). Each nematode was hand-picked and cut for DNA extraction (ref. Peetz and Zasada, 2016). Primers C2F3 and 1108 were used to amplify the mitochondrial DNA COXII (ref. Powers and Harris, 1993). Amplification of the mitochondrial DNA resulted in a single band of ~500 bp. PCR products were cleaned up using the enzyme ExoSap-IT (Thermo Fisher Scientific, USA) and sent for bidirectional sequencing with the same primers used for amplifications at the Oregon State University Center for Quantitative Life Science (Corvallis, OR). The analysis of the sequences resulted in three haplotypes (GenBank accession numbers OQ920919–OQ920921). The BLASTn analysis of these sequences matched with M. hapla with a percentage of identity from 95.1% (accession KP681262) to 100% (accession AY757899). The phylogenetic relationship of M. hapla and other Meloidogyne spp. sequences retrieved from GenBank was estimated using the Bayesian analysis method (ref. Larget and Simon, 1999). The resulting phylogenetic tree placed the M. hapla sequences from hemp in Oregon with other sequences of M. hapla with a posterior probability value of 100% (Fig. 2), supporting the results described above.

These results were also confirmed by using PCR-RFLP and PCR-species specific Intergenic Spacer primers. For PCR-RFLP, the enzyme DRAI (Thermo Fisher Scientific) was used to digest the PCR product obtained with the primers C2F3 and 1108. The restriction pattern consisted of two bands (approximately 200 bp and 250 bp) corresponding to the pattern described for M. hapla (ref. Powers and Harris, 1993). The species was confirmed with a multiplex PCR using the species-specific primers JMV1 (5′-GGATGGCGTGCTTTCAAC-3′), JMV2 (5′-TTTCCCCTTATGATGTTTACCC-3′) and JMVhapla (5′-AAAAATCCCCTCGAAAAATCCACC-3′), which yielded a band of 440 bp (data not shown) corresponding to M. hapla (ref. Wishart et al., 2002).

The pathogenicity of M. hapla on hemp was evaluated under greenhouse conditions. Because of the proprietary nature of many hemp varieties, ‘Sauver haze’ was not available for evaluation in greenhouse studies; therefore, hemp ‘Alpha Explorer’ was considered. Seedlings (5-week-old) of this variety were planted in pots containing 2.1 kg of steam-pasteurized 1:1 sand:loam soil mix. Five hemp plants were inoculated with 4,000 eggs, and five hemp plants were non-inoculated and included as negative controls; three 4-week-old tomato ‘Rutgers’ plants were inoculated with 1,100 eggs as positive controls. The experiment was conducted twice. The experiment was conducted in a greenhouse with average temperatures of 20.8 ± 3.2 °C, and plants were harvested 60 days after inoculation. Eggs from roots and J2 from soil were extracted with the NaOCl and Baermann funnel methods, respectively (ref. Hussey and Barker, 1973; ref. Zasada et al., 2015). Plants did not show any differences in growth based on plant biomass between treatments. No galls were observed on the roots of the inoculated hemp plants. The reproduction factor (RF) was calculated by dividing the final population density by the initial population density. Results from both experiments were combined since there was no statistical difference between the trials (P > 0.05). Meloidogyne hapla successfully reproduced on the tomato positive control (RF = 208.5 ± 18.3), while hemp was a poor host (RF = 0.6 ± 0.1). This result contradicted the high population densities of M. hapla observed in the roots of hemp plants collected from the field. This may be explained by differences in host suitability of hemp genotypes. For example, ref. de Meijer (1993) tested 123 hemp cultivars and reported varying resistance levels to M. hapla, which ranged from highly resistant to moderately susceptible.

This is the first report of M. hapla on hemp in the PNW. Meloidogyne hapla was previously found on hemp in Iowa in 1966 (ref. Norton, 1966). Studies should be conducted to determine pathogenicity of M. hapla on a range of hemp cultivars and the role of hemp as a rotation crop for the management of M. hapla in the PNW.

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