Structural basis for the synergistic assembly of the snRNA export complex
Abstract The nuclear cap-binding complex (CBC) and its partner Arsenite-Resistance Protein 2 (ARS2) regulate the fate of RNA polymerase II…
Torben Heick Jensen
Dual agonistic and antagonistic roles of ZC3H18 provide for co-activation of distinct nuclear RNA decay pathways
Abstract The RNA exosome is a versatile ribonuclease. In the nucleoplasm of mammalian cells, it is assisted by its adaptors…
Temporal-iCLIP captures co-transcriptional RNA-protein interactions
Abstract Dynamic RNA-protein interactions govern the co-transcriptional packaging of RNA polymerase II (RNAPII)-derived transcripts. Yet, our current understanding of this…
Affinity proteomic dissection of the human nuclear cap-binding complex interactome
Abstract A 5′,7-methylguanosine cap is a quintessential feature of RNA polymerase II-transcribed RNAs, and a textbook aspect of co-transcriptional RNA…
NCBP3 positively impacts mRNA biogenesis
Abstract The nuclear Cap-Binding Complex (CBC), consisting of Nuclear Cap-Binding Protein 1 (NCBP1) and 2 (NCBP2), associates with the nascent…
Mapping domains of ARS2 critical for its RNA decay capacity
Abstract ARS2 is a conserved protein centrally involved in both nuclear RNA productive and destructive processes. To map features of…
The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts
Abstract Mammalian genomes are promiscuously transcribed, yielding protein-coding and non-coding products. Many transcripts are short lived due to their nuclear…
Characterizing ZC3H18, a Multi-domain Protein at the Interface of RNA Production and Destruction Decisions
Abstract Nuclear RNA metabolism is influenced by protein complexes connecting to both RNA-productive and -destructive pathways. The ZC3H18 protein binds…
ARS2 is a general suppressor of pervasive transcription
Abstract Termination of transcription is important for establishing gene punctuation marks. It is also critical for suppressing many of the…
Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate
Abstract The nuclear cap-binding complex (CBC) stimulates processing reactions of capped RNAs, including their splicing, 3′-end formation, degradation, and transport.…