Preliminary Evaluation of Two iiPCR Platforms for Qualitative Field Detection of Cannabis sativa

Sydney Hampton, Greta Hundtofte, Rachel Houston

Insulated isothermal PCR (iiPCR) amplifies DNA through convection-based thermocycling and is therefore well suited for field-deployable screening. Here, a commercial iiPCR cannabis assay was evaluated on a benchtop platform (POCKIT Central) and a field-portable platform (POCKIT Micro Duo). The assay targets cannabis DNA rather than THC content and therefore identifies hemp and marijuana alike, matching jurisdictio…