Osteogenic Response of Dental Pulp Stem Cells (DPSCs) to an Alginate/Chitosan/Cannabidiol Hydrogel

Hernan Santiago Garzon, Lina Suárez, Daniel Suárez

This study evaluated the osteogenic differentiation and paracrine response of dental pulp stem cells (DPSCs) cultured within a 3D-printed alginate/chitosan/cannabidiol (CBD) hydrogel. DPSCs from a single donor (passage 6; n = 3 technical replicates per condition) were cultured as monolayers or on hydrogel constructs (CBD 0–12 mg/mL) in basal or osteogenic medium for 7, 14, and 21 days. Alizarin Red S staining conf…