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19172026

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Author: Kazuyoshi Takagi

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Research Article 2019CC BY-NCCC BY-NC allows this article to remain publicly readable here. Reuse is limited to non-commercial use and the recorded source terms.Free full text · non-commercial reuse

Construction of Cellulose Binding Domain Fusion FMN-Dependent NADH-Azoreductase and Glucose 1-Dehydrogenase for the Development of Flow Injection Analysis with Fusion Enzymes Immobilized on Cellulose.

Shigekazu Yano, Yukari Hori, Tatsuro Kijima, Hiroyuki Konno, Wasana Suyotha, Kazuyoshi Takagi +1 more

The cellulose binding domain (CBD) of cellulosome-integrating protein A from Clostridium thermocellum NBRC 103400 was genetically fused to FMN-dependent NADH-azoreductase (AZR) and glucose 1-dehydrogenase (GDH) from Bacillus subtilis. The fusion enzymes, AZR-CBD and CBD-GDH, were expressed in Escherichia coli Rosetta-gami B (DE3). The enzymes were purified from cell-free extracts, and the specific activity of AZR-…

Journal of Applied Glycoscience Europe PMC↗

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