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Author: Jaime E Castellanos

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Brief Report 2022CC BYCC BY allows this article to remain publicly readable here. Any reuse must still follow the recorded source terms.Free full text

Reference gene validation for the relative quantification of cannabinoid receptor expression in human odontoblasts via quantitative polymerase chain reaction.

Laura M Navarro-Saiz, Lilia J Bernal-Cepeda, Felipe García-Jiménez, Deisy Abril, Jaime E Castellanos

Objective The aim of this study was to identify and validate the reference genes in cultured human odontoblasts to quantify their cannabinoid receptor transcripts. Methods The most stably transcribed genes in cultured human odontoblast cells were identified using the RefGenes tool and were selected for real-time polymerase chain reaction (PCR) amplification. Human odontoblast cells were differentiated from mesench…

Odontoblast Rt-qpcr Cannabinoid Receptor 1 Cannabinoid Receptor 2 Reference Gene Rt-qpcr, Reverse Transcription-quantitative Polymerase Chain Reaction Gapdh, Glyceraldehyde‐3‐phosphate Dehydrogenase

Journal of Oral Biology and Craniofacial Research Europe PMC↗

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