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Research Article 2026CC BYFree full text

In Silico Design of phaCAB Expression Constructs for Cellulolytic Hosts Toward Hemp Hurd Valorisation and Polyhydroxybutyrate Biosynthesis

Ziningi Rosebud Myeni, Sani Gumede, Nomfundo Ntombela, Farai Dziike, Nirmala Deenadayalu

Hemp hurds (HHs), an underutilised lignocellulosic biomass (LB) from agricultural waste, offer potential for bioconversion into high-value bioproducts within a circular bioeconomy. Building on prior work involving magnetic nanoparticle-immobilised cellulase hydrolysis of pretreated HH, this study computationally designed candidate phaCAB expression constructs for cellulolytic hosts toward future polyhdroxybutyrate…