Optimization of ultrasound‐assisted solvent extraction of hemp (Cannabis sativa L.) seed oil using RSM: Evaluation of oxidative stability and physicochemical properties of oil

Abstract In this study, the effect of solvent ratio of hexane to isopropanol (0:100, 50:50, and 100:0 v/v%), extraction temperature (30, 45, and 60°C), and time (30, 60, and 90 min) were investigated on the oil extraction efficiency, total phenol content, DPPH radical scavenging, peroxide value, and oil color index. Extraction conditions were optimized by response surface methodology and Box–Behnken design. The optimal conditions were obtained as hexane‐to‐isopropanol ratio of approximately 3:2, temperature of 40.26°C, and ultrasonication time of 54.40