Heat shock protein 90 is an essential molecular chaperone for CB2 cannabinoid receptor–mediated signaling in trabecular meshwork cells

Abstract Purpose: To examine the interaction of heat shock protein 90 (Hsp90) with the CB2 cannabinoid receptor in trabecular meshwork (TM) cells and to investigate the roles of Hsp90 in CB2 receptor–mediated cell signaling and actin cytoskeleton remodeling. Methods: Coimmunoprecipitation experiments and western blot analyses, using specific anti-CB2 and anti-Hsp90 antibodies, were performed to study the interaction of Hsp90 with the CB2 receptor in TM cells. An antiphospho-extracellular-signal-regulated kinases 1/2 (ERK1/2) antibody was used to detect the CB2 receptor–mediated phosphrylation