Combined Administration of Cannabidiol and L-Theanine Improves Sleep-Related Outcomes in Caffeine-Induced Sleep Disturbances Mouse Model

Abstract Sleep disruption is associated with increased risks of metabolic, cardiovascular, and neuropsychiatric disorders. Cannabidiol (CBD) and L-theanine have attracted attention as potential sleep-supporting agents due to their neuroactive properties. This study investigated the individual and combined effects of CBD and L-theanine in a caffeine-induced sleep disturbance mouse model using behavioral assessments and neurochemical analyses. Mice were orally administered CBD and L-theanine at ratios of 1:1, 2:1, or 1:2 prior to pentobarbital-induced sleep testing. Co-administration significantly reduced sleep onset latency