Bromination of Cannabinoids from Cannabis sativa L.: A Comparative Study Using an Enzymatic Catalysis Approach

Abstract An in-depth study on bromination of six typical cannabinoids from Cannabis sativa L. is reported using three different methods: micellar-assisted enzymatic catalysis, NBS, and Oxone. Under the micelle-enabled CiVCPO-catalyzed conditions, selective dibromination of cannabinoids was achieved in up to 72% isolated yield for CBN and 67% for CBL with complete substrate conversion. Notably, the bromination level could be tuned by varying enzyme concentration (300–500 nM), enabling controlled access to mono- or dibrominated products. Compared to conventional methods, the enzymatic