Anti‐Inflammatory Potential of Key Phytochemicals From Humboldtia sanjappae: In Vivo Studies and Molecular Docking With MM‐GBSA Analysis

Abstract Humboldtia sanjappae, an endemic medicinal plant, was examined for its anti‐inflammatory properties using both in vivo and in silico methodologies. The anti‐inflammatory activity was assessed utilizing carrageenan‐induced and formalin‐induced paw oedema models in Swiss albino mice. The ethanolic extract of H. sanjappae demonstrated significant, dose‐dependent inhibition of inflammation. Phytochemical analysis identified bioactive flavonoids, with neoeriocitrin demonstrating the highest binding affinity for TNF‐α in molecular docking studies, indicating its significant role in inflammation modulation. Subsequent docking studies targeting COX‐2, IL‐6,