Comparative Phytochemical Analysis of Five South African Bulbine Species Using Chromatography‐Based Methods

Abstract Bulbine Wolf (Asphodelaceae) comprises over 80 species, with five holding significant medicinal value in South Africa for treating skin and gastrointestinal ailments. While roots are traditionally prioritised, the chemical profiles of leaves and rhizomes remain underexplored although they are also medicinally important. This study used chromatographic and mass spectrometry‐based analytical techniques (HPTLC, LC‐QToF‐MS, and UPLC‐MS) combined with chemometric analysis to chemically profile the metabolites of five Bulbine species from the Eastern Cape. Chemical profiling tentatively identified 73 compounds, including