Differences in hempseed protein separated from AOT reverse micelles and alkaline extraction–isoelectric precipitation

Abstract In this study, Hempseed protein isolates (HPI) and concentrates (HPC) were prepared via bis(2-ethylhexyl) ester sodium salt (AOT) reverse micelle (RMS) and alkaline extraction–isoelectric precipitation (AE) processes, respectively. And their functional properties, structure and nutritional value were compared. RMS-HPI presented a greater protein content but worse protein extraction and recovery rates than AE-HPC did. SDS‒PAGE indicated that AE-HPC dissociated during the separation process, whereas RMS-HPI remained in the natural state. The RMS-HPI was mainly composed of albumin, whereas the