Uncovering mechanism of hepatotoxicity diseases caused by tetrahydrocannabinol based on novel network toxicology and experimental verification

Abstract This study systematically investigated the molecular mechanisms underlying tetrahydrocannabinol (THC)-induced hepatotoxicity in humans through an integrated approach combining network toxicology, molecular docking, and experimental validation. Our analysis identified 22 core targets associated with THC-mediated hepatotoxicity. Protein-protein interaction (PPI) network analysis revealed significant functional associations among these 22 potential target proteins. KEGG pathway and GO term analyses demonstrated that THC potentially exerts hepatotoxic effects through multiple biological processes, including endocrine resistance, bile secretion, negative regulation of apoptosis, and cellular oxidant