Simple and fast quantification of cannabinoids in animal feeds by liquid chromatography–tandem mass spectrometry

Abstract There is growing interest in using hemp materials as animal feed ingredients, which may raise safety concerns because of the potential transfer of active cannabinoids to the resultant products of animal origin. Hence, the detection and identification of cannabinoids in feeds would be useful. We developed a simple, fast, and sensitive method for simultaneous quantification of 4 major cannabinoids in animal feeds by liquid chromatography–tandem mass spectrometry (LC-MS/MS). We used a simple solvent extraction and dilution approach to extract