During the process of Snehapana, since the person was taking only Taila and a very small quantity of food, there is an acceleration of fat utilization for energy in the absence of carbohydrates. This absence of carbohydrate replenishment promotes mobilization of fatty acids from the adipose tissue. Thus, Snehapana has a weight reduction effect on the body. Prior to Snehapana, Sarvanga Udvartana was done which may cause the increase in the lymphatic drainage. It has been proved that lymphatic massage aids in water loss and thus ultimately weight loss.[20]

Gastrium when filled with food inhibits signals to suppress the feeding center; a small quantity of fat is enough to cause this. Fat on entering the gastrium releases cholecystokinin which inhibits further eating. It also causes stimulation of the ventromedial nuclei of the hypothalamus, thus creating complete satiety. Next, when the chyme containing fat enters the duodenum, the activity of the pylorus pump is depressed and the pylorus sphincter is slightly closed. Thus, stomach emptying is slowed.

From the above factors, it is understand that during the process of Snehapana, there is a negative energy balance as it stimulates starvation.[21] This shows the fact that Sneha administered for the purpose of Shodhana impairs the Agni,[22] thus causing a negative energy balance.

Since there is a carbohydrate restriction in the diet, ketosis is induced. Hence, in the process of Snehapana, medically ketosis is induced. This can be explained as in the process of Snehapana, the source of energy is changed to proteins to begin with and then to fat, thus inducing ketosis. After digestion, the excessive fat is lost in the form of steatorrhea. For the purpose of Shodhana Chikitsa, the secretory action of the mucous membrane is exploited.

Here, Moorchita Taila was used for the purpose of Snehapana. The Sneha contains macromolecules of fat and micromolecules of the medicine. During the ingestion, the micromolecules of medicine are absorbed. Another reason is that, in a medicated Taila, the concentration of drugs is far more than the concentration of the drugs seen in other forms of medicines like Kashaya (decoction) or Swarasa (extracted juice of herb).[23]

After the process of Snehapana, Sarvanga Abhyanga was done followed by Swedana in the form of hot water bath. Hot water bath causes hemoconcentration by the process of Sarvanga Vashpa Svedana and it also helps in burning calories.

Virechana is done on the 4^th day. During the process of Shodhana, the body fluids are influenced for therapeutic purposes; here, the body fluids are removed either through the upper or lower route. The GIT is lined by the mucous membrane which has a dual nature of absorption and secretion. The absorption nature is exploited for Shamana Chikitsa and the secretory nature is exploited in Shodhana Chikitsa. During the process of Virechana, cellular fluid is drained into the interstitial fluid, which is drained into the vascular compartment, from here it is drained into the GIT for elimination Thus, Virechana is targeted to create a biochemical alteration as it modulates the fluid compartments of the body.[202122]

During the process of Virechana, about 2 liter of the body fluid is drained out, which has dissolved biochemical in them. Thus, Virechana in the current study has shown its action in three ways. The drugs increase the secretion by irritating the mucous membrane of the GIT. Second, it prevents absorption of nutrients, and finally, it increases the gastro intestinal motility.

Thus, Virechana is targeted to create a biochemical alteration as it modulates the fluid compartments of the body. Further changes over the E. coli colonization were reduced after Virechana and after follow‑up which shows the effect over the gut flora dysbiosis and further induces the stability of gut flora. studies have shown the changes in metabolites across many pathways such as phospholipid biosynthesis, choline metabolism, and lipoprotein metabolism. The observed plasma metabolomics alterations may reflect a Panchakarma‑induced modulation of metabotypes. Panchakarma promotes statistically significant changes in plasma levels of phosphatidylcholines, sphingomyelins and others. Its influence over the immune responses are by regulating pro‑inflammatory cytokines, immunoglobulins and functional properties of T‑cells.[24]