Antioxidant Activities and Oxidative Stabilities of Some Unconventional Oilseeds
Department of Food Technology, Inonu University, 44280 Malatya, Turkey
Department of Chemistry, Inonu University, 44280 Malatya, Turkey
Abstract
The oils of some unconventional oilseeds (hemp, radish, terebinth, stinging nettle, laurel) were obtained by a cold-press method in which the total oil content, fatty acids, tocopherol isomers, some metal contents (Ca, Mg, Fe, Cu), antioxidant activity and oxidative stability were determined. The total oil content was determined ranging between 30.68 and 43.12%, and the oil samples had large amounts of unsaturated fatty acids, with oleic acid and linoleic acid. Of all the oils, terebinth seed oil had the highest α-tocopherol content (102.21 ± 1.01 mg/kg oil). Laurel oilseed had the highest antiradical activity in both the DPPH and ABTS assays. The peroxide value of the non-oxidized oils ranged between 0.51 and 3.73 mequiv O2/kg oil. The TBARS value of the non-oxidized oils ranged between 0.68 ± 0.02 and 6.43 ± 0.48 mmol MA equiv/g oil. At 110 °C, the Rancimat induction period of the oils ranged between 1.32 and 43.44 h. The infrared spectra of the samples were recorded by FTIR spectroscopy. The absorbance values of the spectrum bands were observed and it was determined that some of the chemical groups of oxidized oils caused changes in absorbance. As a result of the present research, the analyzed oils could be evaluated as an alternative to traditionally consumed vegetable oils or as additives to them.
Introduction
Vegetable oils are widely used in cooking and alimentary, as well as the cosmetic, pharmaceutical and chemical industries [1]. Due to the fact that the constituents of non-traditional vegetable oils have unique chemical properties, they are important and may augment other edible oil sources. Some species of the newer sources of edible oils are important because they can be used for both health benefits and the production of formulations and they contain phytochemicals with significant antioxidative properties [2]. Terebinth (Pistacia terebinthus) is used in eczema treatment, asthma and is known for its antibacterial and anti-inflammatory properties [3]. Hempseed (Cannabis sativa), in addition to its nutritional value, has demonstrated positive health benefits, including the lowering of cholesterol and high blood pressure [4]. Radish (Raphanus sativus) is not only a vegetable crop but also an important source of medicinal compounds. Radish is used by people suffering from various gastrointestinal, biliary, hepatic, urinary and respiratory disorders, and in cardiovascular diseases such as hypertension [5]. Stinging nettle (Urtica dioica L.) has been used in the diet and, as a pharmaceutical for a long time [6]. Laurel (Laurus nobilis) is an important plant for many industries. It is widely used in the food, cosmetic, and pharmaceutical industries [7].
On the other hand, oxidation is one of the most common causes of flavor quality deterioration for oils and oil products. Deterioration occurs through rancidity resulting from oxidation which takes place at the double bond sites in the triacyleglycerol molecules. Oxidation causes great economic loses to the food industry [8]. Protection against the oxidation reaction is provided by the tocopherols, phenolic compounds and carotenoids present in the vegetable oils [5]. The addition of antioxidants is method of increasing shelf life of oils and oils products. Synthetic antioxidants, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT), have a restricted use in foods as they are suspected as being carcinogenic [9]. Natural antioxidants are important for human health because of decreasing heart disease risks and possessing anticarcinogenic properties and also they are safer than synthetic antioxidants [10].
The aim of the present study is to determine the fatty acids, tocopherol isomers, some metal contents, the oxidative stability and the antioxidant capacity of selected seeds. These seeds are unconventional seeds and their importance is increased because of their chemical properties. Therefore, they may to be utilized for the food and medical industries due to their antioxidative properties and health benefits.
Materials and Methods
Material
A tocopherol standard (50 mg of α, β, γ, and δ-tocopherol mixture) was purchased from Calbiochem (La Jolla, CA). A fatty acid methyl ester (FAME) mixture (37 component FAME mix) was purchased from Supelco (Bellefonte, PA). All the other chemicals and reagents for analysis were purchased from Sigma (St. Louis, MO).
Sample Preparation
Stinging nettle (Urtica dioica L.), laurel (Laurus nobilis), terebinth (Pistacia terebinthus), hemp (Cannabis sativa), and radish (Raphanus sativus) seeds were purchased from local shops in Malatya, Istanbul, Mersin and Muğla and were blended. The seeds were cleaned and ground. The oils were extracted by using a laboratory type oil-press (Cesalsan, Giresun). Until use, the oils were kept in glass-containers having a −20 °C nitrogen atmosphere.
Total Oil Content
The total oil content of the samples was determined by the AOAC standard methods [11]. A 10-g oil sample was taken from the seeds ground in the coffee mill and the extraction was made with 200 mL n-hexane in a Soxhlet apparatus for 8 h. The solvent was then evaporated at 40 °C by using a rotary evaporator (Bibby Sterilin Ltd, Staffordshire, UK). The above mentioned process was implemented three times for each sample. The average value was obtained and the oil content was expressed as a percentage.
Fatty Acid Analysis
Fifty milligrams of oil was methylated with 3 mL HCl in methanol at 95 °C for 1 h. The fatty acid methyl esters (FAME) were extracted with 2 mL of hexane and dried over sodium sulfate [12]. One microliter of the FAME was analyzed with a Shimadzu GC-17A gas chromatograph (Shimadzu Company, Japan) equipped with a flame ionization detector, and an AOC-20i automatic injector. A SP-2560 capillary column (100 m × 0.25 mm i.d. × 0.2 μm; Cat No. 2-4056) was used. The oven temperature was programmed as follows: 120 °C for 5 min, increased to 240 °C at 4 °C/min, and kept at 240 °C for 25 min. The injector and detector temperatures were each kept at 260 °C. The carrier gas helium, the flow rate 30 mL/min, and the split ratio was 1/50. FAME identification was based on the retention times as compared with those of the standard FAME mixture. Results were expressed as percentage of the peak area without any corrections. Fatty acid analysis was performed in triplicate for each sample, and the average values were reported.
Tocopherol Analysis
Tocopherol composition of the samples was determined as described by Turan et al. [13]. Tocopherols were analyzed by a HPLC system (Shimadzu Prominence, Kyoto, Japan). The normal phase column in the system was an Inertsil NH2 column (250 mm × 4.6 mm, 5 μm) and the column temperature was maintained at 30 °C. Separation of tocopherols was based on isocratic elution with n-hexane (96%) and isopropanol (4%) at 1 mL/min. The eluate was monitored at 292 nm by using a photodiode-array detector (SPD-M20A). The compounds were identified by comparing their retention times and the UV spectra with the authentic standards. Tocopherols were quantified based on the peak areas compared with the external standards. Tocopherol analysis was performed in triplicate for the single samples of each variety, and the average values were reported.
Antioxidant Tests
DPPH Test
The radical scavenging power was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) [14] method. For the DPPH test, DPPH solution was dissolved in a small volume of ethyl acetate and diluted with ethyl acetate by adjusting the absorbance to 0.700 ± 0.020 at 520 nm. A 20-mg oil sample was weighed in a test tube, and 80 μL ethyl acetate as well as 2.9 ml DPPH• free radical solution were added. Next, the sample was agitated with a vortex mixer for 20 s. After 30 min of incubation in darkness, absorbance was measured at 520 nm against ethyl acetate. The results of the DPPH test were expressed as microgram trolox equivalent/g oil. (equivalent to 10 mL methanolic extract).
ABTS Test
The radical scavenging power was determined by the 2,20-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) [15] method. ABTS radical cation (ABTS+) stock solution was produced by reacting 7.0 mM ABTS stock solution with 2.45 mM (final concentration) potassium persulfate in the dark for 16 h. The resulting solution was diluted with ethanol by adjusting the absorbance to 0.700 ± 0.020 at 765 nm. 100 μL diluted oil samples in ethanol and 2.9 mL diluted ABTS+ solution were added. The solution was agitated with a vortex mixer for 20 s. The absorbance was measured after 6 min at 765 nm. The results of the ABTS test were expressed as microgram trolox equivalent/g oil.
Oven Test
Ten grams of oils were weighed in glass Petri dishes (15 mm height and 80 mm diameter) and placed in a forced-draft air oven set at 60 ± 1 °C. Hemp oil (0.5 g) was removed from the oven after 1, 3, 6 days, stinging nettle oil (0.5 g) was removed from the oven after 1, 3, 6, 8 and the oil samples of radish, terebinth (0.5 g) were removed from the oven after 1, 3, 6, 7, 8, 10 days and laurel oil (0.5 g) was removed from the oven after 1, 3, 6, 7, 8, 10, 14, 20, 24 days and flushed with nitrogen, covered with parafilm and kept at −20 °C for the oxidative stability test.
Oxidative Stability
The peroxide value (PV) was determined by the ferric thiocyanate method [9]. A 0.1-g oil sample was weighed and 9.7 ml ethanol added. Thus, the oil was dissolved. Next, 0.1 mL NH4SCN and 0.1 mL FeCl2 were added to the above-mentioned solution, and kept at room temperature for 5 min. Absorbance of the sample was measured at 500 nm. The results were expressed in mequiv/kg oil.
TBARS (2-thiobarbituric acid-reactive substances) was determined as described by Abuzaytoun et al. [16]. A 0.05–0.20-g oil sample was weighed into 25-mL volumetric flasks, dissolved in a small volume of 1-butanol, and made up to the mark with the same solvent. A 5.0-mL portion of this mixture was transferred into a dry test tube, and then a fresh 2-TBA reagent (5 mL of a solution of 200 mg 2-TBA in 100 mL 1-butanol) was added to it. The contents were mixed and heated in a water bath at 95 °C for 2 h. The absorbance of the resultant colored complex was measured at 532 nm. The TBARS values were calculated by multiplying the absorbance reading by a factor of 0.347. This factor was determined from a standard line prepared using 1,1,3,3-tetramethoxypropane as a precursor of malonaldehyde (MA). The results were expressed in mmol MA equiv/g oil.
The induction period of the oil samples were determined by the Metrohm Rancimat apparatus model 743 (Metrohm, Switzerland) [17]. Then, 4.0 g of each oil sample was weighed in the reaction vessel glassware. The conductimetry cells were filled with deionized water up to 90 mL. Samples were heated at 110 °C and air was passed through the heated oil at a rate of 20 L/h. The induction period was determined automatically by the device and expressed in hours.
The infrared spectra of the samples were recorded on a FTIR (Varian 1000 Model) system with a horizontal attenuated total reflectance (ATR) apparatus [18]. The spectrometer was equipped with a deuterated triglycine sulfate detector and purged with dry nitrogen (DuraDry, Haverhill, MA). The ATR crystal was cleaned with pure chloroform before each measurement. A 40-μL oil sample was spread as a thin layer in the ATR crystal and periodical scans (18 scans, 4 cm−1 resolution) were obtained in the spectral range of 400–4,000 cm−1 at 20-min intervals for 360 min. The induction times, i.e. the time needed for a dramatic increase in absorbance, were determined algebraically [18].
Statistical Analyses
Experimental data were evaluated by using analysis of variance (ANOVA) and the significant differences amongst the means of the three replicates (P < 0.05) were determined by Duncan’s multiple range test, using the ‘‘SPSS 9.0 for Windows”.
Results and Discussion
Total Oil Content
Total oil content of seeds is shown in Table 2. The oil contents of these seeds were found ranging from 30.68 to 43.12%. Terebinth and radish seed had the highest oil content (43.12 ± 0.34%, 42.64 ± 1.36%, respectively), followed by laurel seed (36.82 ± 0.36%), hemp seed (31.48 ± 1.19%) and stinging nettle seed (30.68 ± 1.78%) (P < 0.05). In a previous study concerning the hemp seed, the oil content identified and obtained ranged between 25 and 35% [4]. In the present study, the total oil content was identified as 31.48%. In another study concerning the terebinth seed, the total oil content identified and obtained ranged from 35.8 to 43.1% [3]. In the present study, the total oil content was identified as 43.12%. These results agreed with the literature data.
Fatty acid Hemp Terebinth Radish Stinging nettle Laurel Lauric (C12:0) nd nd nd nd 17.31 ± 0.66 Palmitic (C16:0) 7.06 ± 0.11c 23.34 ± 1.03a 4.08 ± 0,33d 7.57 ± 0.04c 17.53 ± 0.02b Stearic (C18:0) 2.77 ± 0.09b 1.51 ± 0.02d 1.81 ± 0.04c 4.11 ± 0.01a 1.80 ± 0.13c Oleic (C18:1) 10.55 ± 0.19d 50.58 ± 0.82a 19.08 ± 1.01c 19.88 ± 0.02c 36.41 ± 0.38b Linoleic (C18:2) 55.48 ± 0.12b 19.88 ± 0.20c 10.09 ± 0.76d 66.37 ± 0.10a 19.06 ± 0.05c Linolenic (C18:3) 21.51 ± 0.14a 1.13 ± 0.01d 7.02 ± 0.91b 0.82 ± 0.01e 1.99 ± 0.01c Heneicosanoic (C21:0) 0.42 ± 0.14b 0.19 ± 0.01b 10.31 ± 1.18 a 0.34 ± 0.01b 0.74 ± 0.19b Erucic (C22:1) nd nd 40.83 ± 1.48 nd nd ΣSFA 11.92 ± 0.07 25.69 ± 0.25 20.61 ± 0.32 12.87 ± 0.01 41.00 ± 0.17 ΣMUFA 10.55 ± 0.19 53.30 ± 0.30 62.28 ± 0.09 19.94 ± 0,02 37.95 ± 0,16 ΣPUFA 77.53 ± 0.09 21.01 ± 0,11 17.11 ± 0.83 67.19 ± 0,06 21.05 ± 0.03 Total oil content (%) 31.48 ± 1.19c 43.12 ± 0.34a 42.64 ± 1.36a 30.68 ± 1.78c 36.82 ± 0.36b
Fatty Acids
The fatty acids of the oilseeds are shown in Table 2. It was determined that these analyzed oils which are very essential for health, are rich in oleic and linoleic acids [20]. The oleic acid content of terebinth seed oil contributed 50.58 ± 0.82% to the total fatty acids, followed by laurel seed oil (36.41 ± 0.38%), stinging nettle (19.09 ± 1.01%), and radish seed oil (15.63 ± 0.04%) (P < 0.05). The linoleic acid content of stinging nettle seed oil contributed 66.37 ± 0.10% to the total fatty acids, followed by hemp seed oil (55.48 ± 0.12%), terebinth seed oil (19.88 ± 0.02%), laurel seed oil (19.06 ± 0.05%), and radish seed oil (10.09 ± 0.76%) (P < 0.05). The linolenic acid content of hemp seed oil contributed 21.5 ± 0.14% to the total fatty acids (P < 0.05). The erucic acid content of radish seed oil contributed 40.83 ± 1.48% to the total fatty acids. In a research aiming to define the components of fatty acids of terebinth seed oil taken from different regions, oleic acid mostly ranged between 43 and 51.3% [3]. In the present research, the oleic acid content of terebinth seed oil is 50.58 ± 0.82%. When the values were compared, it was observed that the present study agreed with the those found in the literature. Likewise, the components of stinging nettle and hemp seed oil fatty acids also complied with the literature [4, 6]. Total saturated fatty acids (SFA) were found ranging from11.92 ± 0.07 to 41.00 ± 0.17%, total monounsaturated fatty acid (MUFA) were found ranging from 10.55 ± 0.19 to 62.28 ± 0.09% and total polyunsaturated fatty acid (PUFA) were found ranging from 17.11 ± 0.83 to 77.53 ± 0.09% (Table 2). For all the oils, total unsaturated fatty acids value (monounsaturated and polyunsaturated fatty acid) was higher than the saturated fatty acids (SFA) value. More saturated fatty acid was determined in laurel seed oil in comparison with the other oils (lauric, palmitic, stearic acids). These oils are basically used for human diets as all the oil samples contain unsaturated fatty acids, such as oleic, linoleic and linolenic acids. Furthermore, linoleic and linolenic acids are known to reduce the incidence of cancer and heart diseases [21]. Thus, terebinth, hemp, stinging nettle, laurel oil seeds may be used in edible oil production.
Tocopherol Content
Tocopherol isomers are shown in Table 3. Terebinth seed oil had the highest α-tocopherol isomer value (102.21 ± 1.01 mg/kg oil) (P < 0.05). Hemp and radish seed oil had the highest γ-tocopherol value (597.91 ± 12.14 and 545.67 ± 15.55 mg/kg oil, respectively). Hemp oilseed had the highest δ-tocopherol value (39.71 mg/kg oil). Laurel seed oil had the highest β-tocopherol isomer value (313.96 ± 0.05 mg/kg oil). Tocopherols are important antioxidant compounds for oils. The oxidative stability of the oils is mostly based on these compounds [22]. Antioxidants are used as lipid stabilizers in the food industry [23]. Moreover, antioxidants have positive effects on oxidation, a destructive force which causes cancer and ageing [2]. Recently natural antioxidants have been preferred rather than synthetic antioxidants, due to health concerns. Therefore, cold-pressed seed oils may be used as food additives for improving food quality and stability.
Hemp Terebinth Radish Stinging nettle Laurel α-Tocopherol 25.58 ± 0.58c 102.21 ± 1.01a 28.66 ± 0.23c 34.96 ± 0.29b 28.96 ± 3.23c β-Tocopherol 5.96 ± 0.05c 3.24 ± 0.23d 12.41 ± 0.72b nd 313.96 ± 0.05a γ-Tocopherol 597.91 ± 12.14a 130.54 ± 8.65d 545.67 ± 15.55b 372.29 ± 1.17c 45.33 ± 0.94e δ-Tocopherol 39.71 ± 1.47a 13.79 ± 1.94b 12.41 ± 0.12b 3.80 ± 0.17c 3.29 ± 0.41c PV 0.90 ± 0.64c 2.13 ± 0.29b 3.73 ± 0.51a 0.85 ± 0.17d 0.51 ± 0.12e TBARS 6.43 ± 0.48a 0.68 ± 0.02e 4.43 ± 0.01b 3.88 ± 0.68c 1.34 ± 0.13d DPPH 62.37 ± 0.32b 52.13 ± 0.75c 52.62 ± 1.16c 46.01 ± 0.22d 85.79 ± 4.81a ABTS 39.69 ± 0.46c 46.53 ± 1.15b 35.57 ± 0.13d 33.18 ± 0.69e 85.28 ± 1.16a
Antioxidant Tests
The DPPH and ABTS radical scavenging capacities are shown in Table 3. The radical-scavenging activity of oils may be influenced by the radical system and other testing conditions. For estimating the radical capacities of antioxidants, stable radicals such as DPPH and ABTS [24] were used. Laurel seed oil had the strongest DPPH-scavenging capacity amongst the five tested oil extracts (85.79 mg trolox/100 g oil), followed by hemp seed oil (62.37 mg trolox/100 g oil), radish seed oil (52.62 mg trolox/100 g oil), terebinth seed oil (52.13 mg trolox/100 g oil), and stinging nettle seed oil (46.01 mg trolox/100 g oil) (P < 0.05). Laurel seed oil had the strongest ABTS scavenging capacity (85.28 mg trolox/100 g oil), followed by terebinth seed oil (46.53 mg trolox/100 g oil), hemp seed oil (39.69 mg trolox/100 g oil), radish seed oil (35.57 mg trolox/100 g oil), and stinging nettle oil (33.18 mg trolox/100 g oil). It was determined that laurel seed oil had the highest activity in both the tests (P < 0.05). It might be due to contents of tocopherol and phenolic compounds Fig. 1.
Oxidative Stability Test
The PV is commonly used to estimate the level of oxidative deterioration in heated oils [25]. The PV of the oils tested in the present study is given in Table 3. The PV of non-oxidized oils ranged between 0.51 and 3.73 mequiv O2/kg oil. The Codex Alimentarius Commission (1982) stipulated a permitted maximum peroxide level of not more than 10 mequiv/kg oil [25]. As a result, the peroxide value of these edible oils was below the acceptable level. The variations amongst the peroxide values in the oils kept in 60 °C oven for oxidation are shown in Fig. 2. The PV values of the oils increased at various ratios. The rise in the PV value of laurel oil continued from day 1 to day 14 and reached its maximum value on the 24th day. In the other oils, the rise of PV was very fast. In hemp oil, the pronounced rise in PV was produced between day 3 and day 5, in stinging nettle oil between day 1 and day 3, and in radish and terebinth oil between day 3 and day 7. Hemp oil is also known to be highly unstable, despite the presence of different minor antioxidant components such as tocopherol that play a dramatic role in oxidative stability [15]. Hemp seed oil has high content of unsaturated fatty acids which are highly susceptible to oxidation. So, we can say that hemp seed oil is more prone to oxidation than others. In contrast, laurel seed oil was more stable than the others. It may be due to a higher content of saturated fatty acids and higher antioxidant activity.
Secondary oxidation products are indicators of oil rancidity. We used the TBARS method that is commonly used to estimate the secondary oxidative products in heated oils [15]. TBARS of the oils tested in the present study is given in Table 3. TBARS values of hemp, stinging nettle, terebinth and laurel seed oil increased over 5, 10, 10 and 24 days, respectively. A sharp increase in TBARS values was noted for radish oil for the first 3 days followed by a decrease and then they increased again. This may be due to volatilization of secondary oxidation products or their break down.
The Rancimat induction periods of oils are shown on Fig. 3. According to the result of the Rancimat analysis, the induction periods of the oils ranged between 1.32 and 43.44 h. Laurel had the highest induction period (43.44 h), followed by, terebinth seed oil (37.55 h), radish seed oil (8.02 h), stinging nettle seed oil (5.57 h), and hemp seed oil (1.32 h) (P < 0.05). The Rancimat method is a powerful and fast technique for estimating the oxidative stability of oils [17, 26]. Laurel and terebinth had higher induction periods than the other oils (P < 0.05). The tocopherol content and unsaturation degree of oil have a significant impact on its oxidative stability [16]. Lower Rancimat induction times were observed for oils containing highly unsaturated fatty acids, such as hemp seed and stinging nettle. The seed oils which have high antioxidant activity and high content of fatty acid were observed to be stable during the oxidation process.
FTIR spectroscopy, used for detecting and quantifying functional groups arising during the oxidative degradation of lipids is a new technique. At present, the characteristic wavenumber regions of the oxidation products are used for observing their formation. Non-oxidative fats have a very low level of hydroperoxide shown as R–O–O–H. During oxidation, the hydroperoxide level increases due to the binding of oxygen to double bonds [27]. The infrared spectrum of non-oxidized stinging nettle oilseed is shown Fig. 4. The overall appearance of the FTIR spectra of non-oxidized edible oils is very similar, but they show differences in absorbance of the bands because of their different composition. Figure 5 illustrates the important spectral changes under the oxidative conditions produced in stinging nettle oil on different days. An increase and decrease in some of the wavenumber regions was observed. However only the regions that were known to be due to certain oil oxidation products were evaluated. The wavenumber region of 700–725 cm−1 belongs to the cis double bonds in unsaturated fatty acids [28]. During oxidation owing to the loss of cis double bonds, the weak band near 1,650–1,700 cm−1 is associated with the stretching vibration of the carbon–carbon double bonds of cis-olefins. The non-oxidized oil samples show the band of the triglyceride ester groups at 1,746 cm−1 [29]. The absorbance increase near the 3,400–3,600 cm−1 region was reported to be caused by the formation of hydroperoxides and alcohols [28]. Generally it is accepted that 3,444 cm−1 is a characteristic band for hydroperoxides [30]. Due to the increase in the hydroperoxide ratio, the bands observed in oxidized oils had higher levels than those observed in unoxidized oils. A summary of various spectral changes and their possible oxidative significance is given in Table 4.
Spectral region Responsible groups 3,444 cm−1
Overtone of the glyceride ester carbonyl absorption [30] 3,006 cm−1
C–H stretching vibration of cis-double bond (=CH) [31, 32] 2,925 cm−1, 2,854 cm−1
Asymmetric and symmetric stretching vibration of the aliphatic CH2 functional group [33] 2,962 cm−1, 2,872 cm−1
Symmetric and asymmetric stretching vibration shoulder of the aliphatic CH3 group [32] 1,743 cm−1
Ester carbonyl functional group of the glycerides [31, 32] 1,654 cm−1
C=C stretching vibration cis-olefins [32] 1,458 cm−1
Bending vibrations of the CH2 and CH3 aliphatic groups [31] 1,397 cm−1
Bending in plane vibration of CH cis-olefin groups[31, 32] 1,373 cm−1
Bending vibration of CH2 groups [31, 32] 1,238 cm−1, 1,163 cm−1
Stretching vibration of C–O ester groups[32] 717 cm−1
Overlapping of CH2 rocking vibration and the out-of-plane vibration of cis-disubstituted olefins [31]
Conclusion
Cold-pressed hemp, terebinth, laurel, and stinging nettle seed oils contain significant levels of polyunsaturated fatty acids which are important to health. Particularly, hemp seed oil has a high level of linolenic acid. Due to the high content of erucic acid, radish seed oil is not used for the food industry, but it can be used in other industries. These oils, except radish seed oils, are utilized as an important source of antioxidants for food. Natural antioxidants are important for health and dietary conditions. Laurel seed oil has a higher antioxidant activity than the other oils. Therefore, it has the longest induction period.
These oils can be blended with common oils, during storage and heating. These oils can also be used to improve the quality, stability and safety of food products. To investigate the other chemical compounds of these cold-pressed oils and their potential biological activities, further research is required.
Acknowledgements
This study was supported by the Inonu University Scientific Research Center (Project No: 2007/42).
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