Potential Neuroprotective Effect of the Endocannabinoid System on Parkinson's Disease
1 Chemical Engineering Department, University Center for Exact and Engineering Sciences University of Guadalajara, Blvd. M. García Barragán # 1451, Guadalajara C.P. 44430, Jalisco, Mexico
2 Cellular and Molecular Neurobiology Laboratory Neurosciences Division Western Biomedical Research Center (CIBO) Mexican Social Security Institute, Sierra Mojada #800, Independencia Oriente, Guadalajara 44340, Jalisco, Mexico
Abstract
Parkinson's disease (PD) is a neurodegenerative disorder characterized by alterations in motor capacity resulting from a decrease in the neurotransmitter dopamine due to the selective death of dopaminergic neurons of the nigrostriatal pathway. Unfortunately, conventional pharmacological treatments fail to halt disease progression; therefore, new therapeutic strategies are needed, and currently, some are being investigated. The endocannabinoid system (ECS), highly expressed in the basal ganglia (BG) circuit, undergoes alterations in response to dopaminergic depletion, potentially contributing to motor symptoms and the etiopathogenesis of PD. Substantial evidence supports the neuroprotective role of the ECS through various mechanisms, including anti-inflammatory, antioxidative, and antiapoptotic effects. Therefore, the ECS emerges as a promising target for PD treatment. This review provides a comprehensive summary of current clinical and preclinical evidence concerning ECS alterations in PD, along with potential pharmacological targets that may exert the protection of dopaminergic neurons.
1. Introduction
Parkinson's disease (PD) is a neurodegenerative disorder predominantly affecting the elderly population. This pathology is mainly characterized by motor symptoms such as rigidity, bradykinesia, tremor at rest, and postural deterioration. Additional symptoms often include loss of balance, hypotension, hyposmia, dysphagia, cognitive impairment, and affective disorders, among others. The main pathophysiological features of PD are the cytoplasmic accumulation of the α-synuclein protein, known as Lewy bodies, and the progressive loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc), resulting in diminished dopamine levels. These changes affect various brain structures, including the cerebellum, the thalamus, the hypothalamus, parts of the cerebral cortex, the spinal cord, the locus coeruleus, and basal ganglia (BG) [1, 2].
Numerous interconnected mechanisms contribute to the degeneration of nigrostriatal dopaminergic neurons, acting simultaneously. These include mitochondrial dysfunction, oxidative stress, proteostasis alteration, inflammation, microglial dysregulation, and excitotoxicity. The study of these mechanisms has identified molecular targets and neurotransmission systems influencing the genesis and progression of PD. This understanding shapes the development of future neuroprotective strategies based on multifactorial pharmacology [3].
The current pharmacological treatments for PD are limited. Levodopa (L-DOPA), the gold standard for advanced-stage symptomatic relief, does not impede disease progression. Long-term L-DOPA use leads to levodopa-induced dyskinesias (LIDs), prompting physicians to initially prescribe less effective dopamine agonists or monoamine oxidase inhibitors, which also fail to halt disease progression and ultimately lead to L-DOPA administration and LID [4]. Consequently, research is ongoing for new therapeutic alternatives to stop or delay PD development while protecting dopaminergic nerve cells to support proper motor function. In this review, we summarize clinical and preclinical findings on the involvement of the ECS through its endogenous ligands, catabolic enzymes, and receptors as potential therapeutics for PD [3, 5].
2. Endocannabinoid System
ECS is a molecular signaling system present in a diverse array of human cells and tissues. It is also present in many other mammals, and its presence in rodents is very suitable due to its wide use in preclinical research. The ECS plays a pivotal role in modulating various physiological functions, including mood, pain, feeding behavior, and motor control, while also contributing to the pathogenesis of several psychiatric and neurological disorders [6]. The ECS is a complex regulatory network comprising endogenous ligands known as endocannabinoids (eCBs), receptors, and diverse proteins responsible for the eCB synthesis, transport, and degradation [3].
2.1. Ligands
Endogenous cannabinoids are lipophilic molecules produced tonically and on demand from lipid precursors in the membrane of postsynaptic neurons and glial cells, responding to physiological and pathological stimuli. In the nervous system, eCBs are recognized for their ability to modulate neural activity, acting also as regulators of the immune response and antioxidant agents, endowing them with neuroprotective properties. These actions depend on their interaction with receptors and are tightly regulated by reuptake (mediated by selective transporters in neuronal and glial cells) and subsequent inactivation through enzymatic degradation [7]. The most abundant and well-described eCBs in the brain parenchyma are 2-arachidonoylglycerol (2-AG) and anandamide (AEA). Together with other ECS components, they significantly regulate motor function within the BG circuit [8].
2.2. Receptors
Two proteins, cannabinoid receptors type 1 (CB1) and 2 (CB2), originating from different genes, have been characterized and confirmed by using crystallography. Both receptors possess seven transmembrane domains and are coupled to G proteins, resulting in predominantly modulatory effects that vary depending on the cell lineage and the type of G protein coupled [9, 10]. These receptors are expressed on the cell membrane of numerous tissues throughout the organism [11, 12]. While other receptors have been described, only CB1 and CB2 are considered canonical and not orphan [12, 13]. Notably, eCB can interact with non-CB1/non-CB2 targets, including transient receptor potential (TRP) channels [14], peroxisome proliferation activated receptor (PPAR)-α and PPAR-γ [15], as well as the G protein-coupled receptor (GPR) 18, GPR55, and GPR119 [7].
2.3. Biotransformation Enzymes
Two additional components of the ECS are biosynthesis and degradation enzymes. Unlike classical neurotransmitters and neuropeptides, eCBs are not stored in vesicles within axon terminals; instead, they are synthesized on demand in cell bodies and dendrites. They are subsequently released and exert immediate action as signaling molecules. Endocannabinoids are endogenous signaling lipid molecules generated from phospholipid precursors in the cell membrane, primarily derived from arachidonic acid conjugated with ethanolamine, glycerol, other fatty acids, and even neurotransmitters [16, 17].
The principal eCBs include anandamide (AEA, arachidonyl ethanolamine) and 2-arachidonylglycerol (2-AG). The eCB family also encompasses virodhamine, noladineter, N-arachidonoyl dopamine (NADA), homo-γ-linolenylethanolamide (HEA), docosatetraenoylethanolamide (DEA), and related compounds such as palmitoylethanolamide (PEA) and oleoylethanolamide (OEA) [11, 18, 19]. The synthesis of 2-AG primarily involves activating the phospholipase Cβ/diacylglycerol lipase (PLCβ/DGL) pathway. The DGL enzyme coexists in two isoforms, DGLα and DGLβ, with DGLα being the dominant isoform in producing 2-AG within the brain parenchyma in most regions. However, recent work carried out by Liu et al. has demonstrated that DGLβ is particularly relevant in the SNpc, substantia nigra pars reticulata (SNpr), and striatum, uncovering changes in the gene coding for this isoform in some hereditary cases of PD. This discovery led to the generation of a new PD model through conditional knockout of this gene, specifically in the SN [20]. In contrast, AEA is primarily synthesized via N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) in conjunction with other N-acylethanolamides such as oleylethanolamide (OEA) and palmitoylethanolamide (PEA). Regarding eCB catabolism, monoacylglycerol lipase (MAGL) is the predominant enzyme degrading 2-AG to arachidonic acid (AA) and glycerol. In contrast, fatty acid amide hydrolase (FAAH) largely catalyzes the degradation of AEA, OEA, and PEA to ethanolamine and the corresponding fatty acid (AA, oleic acid, and palmitic acid, respectively) [7].
2.4. Transport Mechanism
MAGL, FAAH, and other enzymes responsible for the degradation of eCB are localized within the intracellular compartment, suggesting the existence of cannabinoid transporters. While crystallography has not yet demonstrated the presence of a transporter, evidence supporting its existence arises from the discovery of a transcription variant of the FAAH gene that retains its complete structure except for the catalytic site, coupled with the activity of specific molecules that can only be explained by the involvement of transporter proteins [21]. A brief scheme of the main components of the ECS centered on ligands and their targets is presented in Figure 1.
Current evidence, recapitulated from studies on patients with PD and animal models of parkinsonism, indicates that ECS undergoes modifications within the BG circuit. These alterations have been investigated regarding changes in receptor expression and the activity of biosynthesis and degradation enzymes, leading to variations in ligand levels when comparing healthy and affected individuals. Some of these cases likely represent compensatory mechanisms following dopamine depletion, such as the case of AEA in the striatum. Others are more likely related to the manifestation of motor symptoms. These observations provide a rationale for various methodological approaches to enhance ECS signaling, which have demonstrated positive antiparkinsonian effects [5]. Consequently, the ECS is considered a significant pharmacological target to alleviate motor symptoms and inhibit neurodegeneration progression. It is noteworthy that the distribution of cannabinoid receptors, their neuromodulatory mechanisms, including anti-inflammatory and antioxidant properties, and their role in the pathophysiology of PD have been the primary focus of cannabinoid system research, as will be described later.
3. Alterations of the Endocannabinoid System in Parkinson's Disease
It is well established that eCBs are highly expressed in the BG, and their levels are altered in individuals with PD and animal models of parkinsonism induction. Consistent with this, a clinical study showed that in cerebrospinal fluid (CSF) samples from PD patients, AEA levels were twice as high as in controls, with no correlation between this increase and disease progression. Furthermore, these changes did not manifest in patients undergoing pharmacological treatment with L-DOPA or dopamine agonists [22]. Additionally, studies conducted by Marchioni et al. in 2020 demonstrated reduced levels of 2-AG and increased levels of AEA in CSF of PD patients under L-DOPA treatment without LID, compared to healthy controls. Notably, these alterations were not modified in patients with LID [23]. Also, preclinical studies in animal models of parkinsonism induction have exhibited alterations in the levels of these eCBs in the BG circuit in a region-dependent fashion.
In the striatum of monkeys treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a neurotoxin causing dopaminergic cell death, both AEA and 2-AG levels were increased and reverted to basal levels after levodopa treatment, without correlation with the development of LID [24]. Di Marzo et al. in 2000 demonstrated an increase in 2-AG levels in the globus pallidus of rats treated with reserpine, suggesting that an increase in 2-AG levels might be a consequence of more significant activity in the BG indirect pathway [8]. Moreover, in the striatum of 6-hydroxydopamine (6-OHDA)-lesioned rats, elevated AEA levels were found due to reduced activity of the anandamide membrane transporter and the degradation enzyme FAAH. This led to an overactivation of spontaneous glutamatergic activity [25]. These changes were reversed entirely after chronic L-DOPA treatment, suggesting that the increased AEA levels imply a compensatory mechanism for abnormal glutamatergic input [26].
In a series of studies conducted by Concannon et al. employing various methodologies for parkinsonism induction, alterations in eCB levels in the striatum differed amongst models. AEA levels increased in the striatum of rats lesioned intrastrially with rotenone and lipopolysaccharide (LPS), but this effect was not observed in animals receiving 6-OHDA. Additionally, 2-AG levels were elevated only in animals treated with LPS, while the content of PEA and OEA was increased in all models, albeit in different concentrations. Therefore, special care should be taken when interpreting the results [27, 28].
A recently published study by Liu et al. demonstrated that, in SNpc neurons, the DGLβ isoform predominates in 2-AG synthesis and that the loss of function of this enzyme, induced by genetic mutations, is associated with familial forms of early-onset autosomal recessive parkinsonism. In the same study, the authors showed that pharmacological inhibition of the enzyme MAGL improves the motor response of rodents with a specific deletion of the Dglβ gene in dopaminergic neurons of the SNpc by enhancing the activity of dopaminergic neurons and the consequent release of dopamine [20]. Additionally, Navarrete et al. observed a decrease in MAGL in the SNpc and an increase in striatum samples of PD patients [29].
Regarding cannabinoid receptors, in vivo studies in patients with PD revealed decreased CB1 receptors in the SNpc, not correlated with disease duration [30]. In animal studies, Walsh et al. explored the temporal and regional patterns of CB1 receptor expression in rats unilaterally lesioned with 6-OHDA. CB1 levels did not change in the striatum but were diminished in internal globus pallidus and SNpr. In contrast, in external globus pallidus, the expression levels were increased, although not sustained over time [31]. In postmortem studies, CB2 receptors showed an increased expression in astrocytes of the SNpc [29].
Several experimental pieces of evidence have demonstrated the pivotal involvement of astrocytes and microglia cells in the genesis of PD. During the early stages of the disease, research has shown that astrocytes absorb altered α-synuclein from axon terminals [32], leading to neurodegenerative processes through the production of proinflammatory cytokines [33] and microglial activation [34]. Activated microglia further contribute significantly to the loss of dopaminergic neurons of the SNpc and dopaminergic denervation in the striatum [35, 36]. Moreover, various preclinical and clinical evidence has demonstrated the presence of the CB2 receptor in microglia cells and astrocytes [37], suggesting a neuromodulatory role in both pro- and anti-inflammatory events in PD. Furthermore, Price et al. demonstrated an increase in CB2 receptor expression in microglia cells after administering MPTP. Agonism of the CB2 receptor with JWH015 (4 mg/kg, intraperitoneal) reduced MPTP-induced microglial activation, while genetic ablation of CB2 receptors exacerbated MPTP systemic toxicity. A recent study also reveals a significant increase in gene transcription and CB2 protein expression after intrastriatal injection with 6-OHDA or bacterial LPS. This increase is accompanied by enhanced microglial expression and activation in the SNpc and the striatum, suggesting that the CB2 receptor is a potential pharmacological target to inhibit both inflammatory and oxidative stress events [27].
Similarly, Concannon et al. employing different models of parkinsonism induction reported an upregulation of CB2 receptors in rats' SNpc. This response was slightly more pronounced when an inflammatory stimulus such as LPS was used compared to 6-OHDA and rotenone [28].
4. Evidence of the Neuroprotective Effect of the Endocannabinoid System on Parkinson's Disease
4.1. Endocannabinoids
The neuroprotectant effect of eCBs has been thoroughly examined by various authors. These studies have revealed that eCBs exert anti-inflammatory, antioxidative, and antiapoptotic effects while inhibiting mitochondrial dysfunction and excitotoxicity-induced death, mechanisms implicated in the pathophysiology of PD [3].
Regarding the neuroprotective capacity of 2-AG, a study conducted by Mounsey et al. in 2015 observed that treatment with MPTP (30 mg/kg, i.p. ×5 days) increases the levels of 2-AG in the midbrain of C57BL6/J mice from the second day of administration, maintaining elevated levels until the end of the treatment. This increase in 2-AG, as suggested by Mounsey et al., might be a compensatory mechanism for counteracting the oxidative damage induced by MPTP to prevent further harm. In line with this, the authors demonstrated that pretreatment with 2-AG (3 and 5 mg/kg, i.p.) prevented the death of dopaminergic neurons in the SNpc of MPTP-treated mice. This protective effect was shown to be potentiated by inhibiting the degradation of this eCB [38]. Furthermore, studies have indicated that direct manipulation of 2-AG levels by inhibiting their degradation not only results in symptomatic relief [20, 39–41] but also exerts a neuroprotective effect [38, 42–44].
Preclinical studies demonstrated that treatment with JZL184, a MAGL inhibitor, increases 2-AG levels and prevents the death of neuronal cells of the nigrostriatal pathway at doses between 8 and 40 mg/kg [38, 43]. However, high doses or prolonged use of JZL184 can inhibit the enzyme FAAH [45]. While this represents an additional pathway for 2-AG degradation, FAAH exhibits a higher affinity for AEA and other fatty acid ethanolamines. Consequently, inhibiting FAAH catalytic activity may lead to an increase in the levels of these eCBs [43]. Notably, Fernández-Suárez et al. demonstrated that FAAH inhibition does not prevent the toxicity induced by MPTP plus probenecid (MPTPp), aligning with results from other research groups [40, 41]. Treatment with JZL184 increases 2-AG and other eCB levels, making it difficult to determine if the neuroprotective effect should be attributed solely to 2-AG. In 2017, Pasquarelli et al. evaluated the impact of MAGL inactivation with the inhibitor KML29 (10 mg/kg, i.p.) in C57BL/6J mice undergoing chronic treatment with MPTPp (10 mg/kg and 250 mg/kg, i.p., respectively). The results demonstrated an improvement in motor response and a reduction in dopamine depletion in the striatum. However, it is noteworthy that the authors did not assess the extent of dopaminergic neuronal death in the SNpc [41].
The mechanisms underlying the neuroprotective role of 2-AG against dopaminergic neuron death have received limited attention. In a study conducted by Aymerich et al. in 2016, inhibition of MAGL with JZL184 (0.1 and 1 μM) was shown to counteract the toxicity induced by 1-methyl-4-phenylpyridinium (MPP+: the putative toxic metabolite of the neurotoxin MPTP) in the SH-SY5Y cell line. Notably, this effect was found to be dependent on CB2 receptor agonism. The study revealed that JZL184 increased mRNA levels of CB2 receptors, and the antagonism induced by the addition of AM630 (1 μM) completely inhibited the neuroprotective effect of JZL184 (0.1 μM) [46]. Data collected by Nomura et al. in 2011 demonstrated that both genetic (Mgll −/− mice) and pharmacological (pretreatment with JZL184, 40 mg/kg, v.o.) inactivation of MAGL in an acute MPTP-induced parkinsonism model (15 mg/kg, i.p.) led to reducing levels of AA, prostaglandins, and proinflammatory cytokines. This suggests that blocking 2-AG degradation contributes to decreased inflammatory response triggered by dopaminergic neuron death [42]. Consistent with this, Mounsey et al. demonstrated that inactivation of the cyclooxygenase-2 enzyme (an enzyme involved in the conversion of AA to prostaglandins) with a selective inhibitor of inflammatory mediator cyclooxygenase-2: DFU (25 mg/kg, i.p.) potentiated the neuroprotective effect of MAGL inhibition [38].
Furthermore, the Fernández-Suárez research group observed increased immunoreactivity of microglia and astrocytes in the SNpc and striatum, along with elevated expression levels of transforming growth factor-β (TGF-β) and glial cell line-derived neurotrophic factor (GDNF) in the striatum of mice with induced parkinsonism [43]. These findings suggest that the neuroprotective and antiparkinsonian effects achieved through MAGL inhibition are associated with an anti-inflammatory response mediated by increased 2-AG levels and its interaction with CB2 receptors.
The current evidence on the neuroprotective effect of AEA in animal models of parkinsonism is limited and primarily based on observations following the inhibition of its enzymatic degradation. Furthermore, the mechanisms underlying the protective activity of the nigrostriatal pathway remain unclear. Escamilla-Ramirez et al. employed a potent irreversible FAAH inhibitor, URB597, to increase AEA tone in C56BL/6NHsd mice with acute MPTP-induced parkinsonism (40 mg/kg, s.c.). Results showed that treatment with URB597 (0.3 mg/kg, i.p., ×5 days), before or after neurotoxin administration, improved motor performance and reduced oxidative damage by preventing impairment of the nigrostriatal pathway [44]. Similarly, Viveros-Paredes et al. reported that 30-day pretreatment with URB597 (0.2 mg/kg, i.p., administered every other day) counteracted nigrostriatal pathway denervation, damage-associated immune response, and motor deficit by preventing neuronal death induced by MPTP (30 mg/kg, i.p., ×5 days) [47]. Conversely, Celorrio et al. found that chronic administration of URB597 (1 mg/kg, i.p., five times/week during 5-week MPTPp treatment) did not prevent dopaminergic degeneration. However, AEA degradation blockade improved motor function in mice with hemiparkinsonism [40].
FAAH is also implicated in the degradation of other members of the fatty acid ethanolamide family. URB597-induced FAAH inactivation significantly elevated levels of AEA, OEA, and PEA in the midbrain of mice with parkinsonism [40]. OEA has been described as an agonist at PPAR-α and differs from AEA by functioning as an antagonist of transient receptor potential vanilloid 1 (TRPV1) and lacking interaction with CB1 or CB2 receptors [48]. Gonzalez-Aparicio et al. investigated the protective effect of OEA at different concentrations (0, 5, 1 y 5 mg/kg) in rats lesioned with 6-OHDA (5 μg/μl intrastriatal). Their results demonstrated that intraperitoneal injection of OEA could penetrate the blood-brain barrier, reach active concentrations in the BG, and elicit anti-inflammatory/antioxidant responses. This resulted from the activation of PPAR-α receptors expressed by dopaminergic neurons in both the SN and the dopaminergic fibers in the dorsal striatum [49–52]. PPAR-α receptor activation decreases neuronal death through vascular protection and exerts vascular effects by reducing oxidative stress and preventing adhesion protein expression [53]. PPAR-α also regulates apoptosis and inflammation, inhibiting the transcription of inflammatory response genes by antagonizing the AP-1 and nuclear factor κB signaling pathways (NFκB) [54–56].
The investigations into PEA's neuroprotective and antiparkinsonian effects have mainly focused on its anti-inflammatory capacity, involving the activation of PPAR-α receptors. Esposito et al. evaluated the systemic administration of PEA (10 mg/kg, i.p., ×7 days starting 24 h postinsult with MPTP (80 mg/kg, i.p.)) in parkinsonian mice with PPAR-α gene deletion. Mice presented slightly more significant damage than induced by MPTP, and PEA treatment lost its beneficial effect, indicating that signaling mediated by PPAR-α activation is involved in neuroprotection mechanisms. In the same study, authors observed that PEA prevented apoptosis by modulating the Bax/Bcl-2 pathways, mechanisms largely dependent on the activation of PPAR-α [57]. Similarly, Cupri et al. assessed a micronized formulation of PEA (mPEA, 10 mg/kg, p.o. ×60 days) in aged CD1 mice (21-month-old). Old mice presented more significant cellular and behavioral impairment due to MPTP toxicity than young mice (3 months). mPEA pretreatment demonstrated a decrease in apoptotic death of SNpc neurons, an improvement in motor skills, a reduction in α-synuclein expression, and modulation of proinflammatory (TNF-α and IL-1β) and anti-inflammatory (IL-10) cytokines [57, 58]. Furthermore, the protective and antiparkinsonian effect of PEA described in the MPTP model was corroborated in 6-OHDA partially injured mice (4 μg). Results showed reductions in oxidative stress, inflammation, endoplasmic reticulum stress, and apoptosis at the three doses (3, 10, and 30 mg/kg, s.c., ×8 days). The involvement of PPAR-α receptors was evidenced in vivo with GW7647 administration, a synthetic agonist (5 mg/kg, s.c.) exerting similar effects in every parameter, and in vitro in the SH-SY5Y cell line in which the addition of GW6471, a PPAR-α antagonist (0.3 and 1 μM), prevented the protective effect of PEA (1 μM) [59].
PEA degradation is also catalyzed by the enzyme N-acylethanolamine acid amidase (NAAA). In a recent study, researchers found an increase in NAAA levels in the cerebral cortex of postmortem patients with PD and exosomes derived from the blood of individuals in stages I and II of this disease [60]. Consistent with these findings, in vitro experiments demonstrated increased NAAA expression and decreased PEA concentration in SH-SY5Y cells stimulated with 6-OHDA (100 μM). Interestingly, the addition of MPP+ (2 mM) stimulated enzyme expression but did not lead to a significant decrease in PEA content. In vivo, intrastriatal injection of 6-OHDA (6.4 μg) increased NAAA immunoreactivity in dopaminergic neurons of the SNpc within the initial 48 hours and, two weeks later, in microglial cells confined to the SNpc and striatum.
Moreover, genetic and pharmacological inactivation of NAAA (twice daily administrations of ARN19702 at a dose of 30 mg/kg, i.p.) produced a neuroprotective and antiparkinsonian effect in the lesion model with 6-OHDA and toxicity induced by MPTP (72 mg/kg, i.p.). This study also demonstrated the involvement of PPAR-α receptors in the protective action of PEA. Specifically, the activation of these receptors promoted the survival of dopaminergic neurons, at least partially, by preserving their bioenergetic potential through normalizing the expression of the transcription coactivators PPAR coactivator (PGC)-1α and PGC-1β, which are suppressed by 6-OHDA. The combined evidence from Palese et al. coupled with previously described findings on the protective effect of PEA positions the NAAA-PEA-PPAR-α pathway as a promising therapeutic target for managing PD [60].
4.2. CB2
Under physiological conditions, CB2 receptors are expressed at low concentrations in microglia, astrocytes, and neurons of the hippocampus, striatum, brainstem, ventral tegmental area, and SNpc. However, under pathological conditions, such as PD, their expression predominantly increases in the glial cell lineage [28, 61]. This upregulation is interpreted as an endogenous protective response aimed at delaying or halting the progression of the disease by controlling the inflammatory response. Therefore, pharmacological manipulation of CB2 receptors represents a promising strategy for PD [62]. Several preclinical studies have reported that natural and synthetic agonists for CB2 receptors protect dopaminergic neurons and prevent motor dysfunction, possibly by reducing damage-related glial alterations [62–64]. These findings are supported by evidence that genetic ablation of the CB2 receptor exacerbates the toxicity of MPTP [63].
Regarding the mechanism by which CB2 agonism exerts neuroprotection, a recent study by Wang's group investigated the role of nuclear factor erythroid 2-related factor 2 (Nrf2) in the immunomodulatory effect induced by JWH133, a CB2 agonist, in a microglia culture treated with MPP+. The results indicated that the PI3K/Akt/Nrf2 pathway is involved in the polarization of microglia toward an anti-inflammatory phenotype [65]. Additionally, Yu et al. reported that the neuroprotective effect of GW842166x, another CB2 agonist, in the rodent MPTP model is associated with the capacity of CB2 receptors localized in dopaminergic neurons to reduce the action potential firing and the associated calcium overload that may cause cytotoxicity [64]. The main components of ECS and their alteration in patients with PD and some animal models for the pathology are presented in Table 1.
4.3. PPAR
PPAR is a nuclear receptor group that functions as transcription factors and requires the transcription coactivator PGC-1α. Their activation entails physiological activities primarily mediated by binding to peroxisome proliferation response element sites [67]. These receptors are divided into three subtypes: α, β/δ, and γ; their predominant cellular distribution in brain parenchyma is neuronal. Under physiological conditions, all three isotypes are present in astrocytes, but only isoform α is expressed in microglial cells in both human and mouse brains. However, in response to LPS, PPAR-γ increases in these cells region-dependent, while PPAR-α levels are maintained [68].
Various specific agonists for these receptors have been developed and utilized, especially for PPAR-γ. One of the most important is pioglitazone which has been associated with protective effects against PD in patients and has been already used for other diseases, suggesting that sustained receptor activation could be neuroprotective [69]. Pioglitazone has been effective in the treatment of neurodegenerative disorders at a preclinical level in nonhuman primates [70] and parkinsonism models [69]. Mechanisms underlying the antiparkinsonian effects of this activation have been explored. On one hand, pioglitazone has been proven to regulate neural plasticity and exhibit neuroprotective effects through anti-inflammatory and antioxidative mechanisms [69]. Regarding work in nonhuman primates, transient regulation of paraoxonase-2 expression has been described as a possible mechanism of neuroprotection due to PPAR-γ activation. This protein, widely expressed in regions with a high dopamine concentration, enhances coenzyme Q function at the mitochondrial level, potentially reducing oxidative stress [70]. On the other hand, molecules related to cannabinoids with activity solely on PPAR-γ receptors, such as cannabigerol quinone derivatives VCE-003.2 and VCE-004.8, have been produced [71, 72]. These compounds have exhibited anti-inflammatory effects along with neuroprotective effects and improvement of locomotor activity in parkinsonism models [15, 71–73]. In all these studies, these effects were explained by PPAR-γ activation; nevertheless, an antagonist of these receptors failed to fully deplete its activity, suggesting binding of the derivate in noncanonical regions [73].
4.4. TRPV1
TRPV1 is a nonselective cation channel activated by various endogenous and exogenous stimuli, including heat, natural vanilloids (capsaicin and resiniferatoxin), and eCB, such as AEA [74]. This receptor is widely expressed in sensory neurons, dopaminergic cells, astrocytes, and microglia within the nigrostriatal pathway. Its physiological role involves the modulation of pain perception, motor control, and inflammation [14]. Preclinical studies have shown that agonism of the TRPV1 receptor with capsaicin, a natural agonist, prevents the degeneration of dopaminergic cells in models of parkinsonism induction by inhibiting the inflammatory response. Specifically, it modulates microglia cells from a proinflammatory to an anti-inflammatory immunophenotype [75]. In the MPP+ rat model, Nam et al. showed that the neuroprotective effect of TRPV1 activation is mediated by ciliary neurotrophic factor (CNTF), released by astrocytes, and its receptor (CNTFRα) localized in dopaminergic neurons. Additionally, patients with PD exhibit increased expression of the TRPV1 receptor and CNTF, suggesting the TRPV1 receptor as a therapeutic target for PD [76].
Furthermore, it has been demonstrated that the neuroprotective effect of capsaicin may result from an interaction between the TRPV1 receptor and cannabinoid receptors. This is supported by the observation that the pretreatment with AM251 (CB1 antagonist, 0.1 mg/kg, i.p.) and AM630 (CB2 antagonist, 0.1 mg/kg, i.p.) partially reversed the neuroprotective effect of capsaicin (0.5 mg/kg, i.p.) in mice subjected to acute treatment with MPTP (80 mg/kg, i.p.) [77].
4.5. GPR55
The GPR55 receptor is coupled to the activation of the Gα12/13 and Gαq proteins, whose activation promotes the release of intracellular calcium (Ca2+) from the endoplasmic reticulum and the activation of the ERK pathway. This pathway modulates cellular parameters such as proliferation, differentiation, cell migration, and physiological processes like blood pressure, bone density, inflammation, and energy balance and exhibits antiepileptic action [78]. Due to its abundant expression in the striatum, the GPR55 receptor appears to play a role in the modulation of motor function [79]. Studies conducted by Celorrio et al. demonstrated that GPR55 receptor activation, through agonism with abnormal cannabidiol (a synthetic isomer of cannabidiol with high affinity towards GPR55), prevents dopaminergic cell death, motor deficits, and damage-associated morphological changes in microglia in a chronic MPTPp model in C57BL/6 mice [80]. Additionally, agonism of the GPR55 receptor with VCE-006.1 has been shown to reverse motor deficit induced by 6-OHDA in pole test and cylinder rearing tests, as well as prevent the loss of dopaminergic neurons and inhibit the reactivity of glial cells in SNpc [81]. However, the signaling pathways associated with these neuroprotective effects remain unknown.
It is crucial to note that the antiparkinsonian effects of the GPR55 receptor have been controversial. In a study conducted by Fatemi et al. in 2021, using a model of hemiparkinsonism induced by 6-OHDA, intrastriatal administration of GPR55 agonists (LPI, 1 and 5 μg) and antagonists (ML193, 1 and 5 μg) caused an improvement in motor coordination and attenuated sensorimotor deficits in rats. This suggests that GPR55 may have a modulatory role in PD and provides new insights into its involvement in controlling balance, movement, and sensorimotor skills [82].
5. Neuroprotection on Clinical Evidence?
The neuroprotective activity of the ECS has not been assessed directly in clinical assays, if we consider a strict, cellular biology-oriented meaning of the term. In part given the technical and ethical complexity of measuring such a feature on humans (it would be necessary to assess dopaminergic viable cell numbers in vivo in humans without or with very little adverse effects), there are very few clinical trials that search for this niche definition of neuroprotection [83]. Nevertheless, the consideration of a more broad, operational-oriented definition of neuroprotective activity as the slowing or halt of neurological disease features and symptoms leads us to point out several studies involving diverse drugs targeting the ECS. Regarding this, there are some works that highly emphasize the evidence on the enhancement of ECS signaling, postulating the benefits for targeting it with diverse pharmacological approaches affecting variated targets related to, or that are part of the system itself abovementioned. In Table 2, we summarize their findings along with the components of the ECS presumably affected by the drugs of intervention.
6. Conclusions
Considering current evidence, the ECS emerges as a promising therapeutic target for managing PD, primarily owing to its neuroprotective effects, prominently mediated through anti-inflammatory mechanisms. This is particularly significant since neuroinflammation stands out as a hallmark of PD, and extensive preclinical studies have consistently demonstrated that modulating this inflammatory process mitigates the progression of dopaminergic neuronal death. However, the mechanism underlying how the ECS exerts neuroprotection and anti-inflammatory/immunomodulatory actions remains poorly described, highlighting the need for more comprehensive studies on this matter. Concerning the neuroprotective effect of CB1 receptors in parkinsonism models, the evidence is limited, contradictory, and inconclusive, particularly regarding elucidating the involved mechanisms. Further research is needed to address these gaps and provide a clearer understanding of the specific ways in which the ECS contributes to neuroprotection in the context of parkinsonism.
Acknowledgments
This work was financially supported by Consejo Nacional de Humanidades, Ciencia y Tecnología (CONAHCyT) project (# 281452) granted to Dr. Mario Eduardo Flores-Soto and Scholarship (# 663489) granted to Maria Fernanda Urmeneta-Ortíz.
Data Availability
Any information regarding the contents of this paper will be properly disclaimed by the correspondence author upon reasonable request.
Conflicts of Interest
The authors declare that there are no conflicts of interest regarding the publication of this paper.
| Type and component of the ECS | Alteration | Experimental subject/model/sample | References | |
|---|---|---|---|---|
| Ligands | AEA | Augmentation | CSF of patients | [22, 24] |
| Monkeys and rats | [66] | |||
| Animal models | [27, 28] | |||
| 2-AG | Diminishment | CSF of patients | [23] | |
| Augmentation | Rat 6-OHDA and reserpine models | [8, 27] | ||
| OEA | Augmentation | Animal models | [27, 28] | |
| PEA | Augmentation | Animal models | [27] | |
| Diminishment | Rat 6-OHDA model | [28] | ||
| Mice model | [60] | |||
| Production enzymes | NAAA | Augmentation | Rat 6-OHDA model | [60] |
| Mice model | ||||
| DAGL | Loss of function | Patients (hereditary Parkinson's) | [20] | |
| Gene KO | Mice model | |||
| Degradation enzymes | FAAH | Decreased activity | Rats 6-OHDA model | [25] |
| MAGL | Augmentation | Brains postmortem | [29] | |
| Receptors | CB1 | Diminishment | Blood of patients | [30] |
| +/− | Rats 6-OHDA model | [31] | ||
| CB2 | Augmentation | Animal models of PD | [28, 61, 62] | |
| Components of the ECS targeted | Substance(s) employed, dosage, and administering route | Effects observed | References |
|---|---|---|---|
| CB1 and CB2 agonism | Nabilone 0.03 mg oral | Reduction of rush dyskinesia disability, painful dystonia, and time | [84] |
| Inverse agonist to CB1 | Rimonabant 20 mg oral | No improvement of motor symptoms | [85] |
| All components on ECS affected and many other targets | Whole cannabis smoked | Subjective improvement of rest tremor bradykinesia and dyskinesias | [86] |
| All components on ECS affected and many other targets | Cannador oral capsule (ethanolic extract containing 2.5 mg of delta-9-tetrahydrocannabinol (THC) and 1.25 mg of cannabidiol (CBD)) | No improvement in dyskinesias | [87] |
| CB1 and CB2 agonism, FAAH inhibition, and PPAR and TRPV modulated activities | CBD 150 mg oral | Reduction of psychotic symptoms | [88] |
| All components on ECS affected and many other targets | Whole cannabis smoked | Improvement on reducing tremors, rigidity, and bradykinesia, as well as sleep and pain | [89] |
| CB1 and CB2 agonism, FAAH inhibition, and PPAR- and TRPV-modulated activities | CBD 75 and 300 mg oral | Reduction of disorder related REM sleep behavior | [90] |
| CB1 and CB2 agonism, FAAH inhibition, and PPAR- and TRPV-modulated activities | CBD 75 and 300 mg oral | No improved motor but improved in quality of life on highest dosage | [91] |
| All components on ECS affected and many other targets | Cannabis different dosage (patient survey) | Improvement of mood and sleep and of motor symptoms | [25, 92] |
| CB1 and CB2 agonism | Nabilone 0.25 mg to 1 mg oral | Beneficial effects on sleep disorders associated with PD | [93] |
| CB1 and CB2 agonism, FAAH inhibition, and PPAR- and TRPV-modulated activities | CBD 300 mg oral | Decrement in anxiety and tremor amplitude on PD patients | [94] |
| CB1 and CB2 agonism | Nabilone 0.25 mg to 2 mg oral | Beneficial effects on sleep outcomes in PD patients | [95] |