Structural Basis for Multiple Sugar Recognition of Jacalin-related Human ZG16p Lectin*

Abstract Background: ZG16p is a soluble mammalian lectin with a Jacalin-related β-prism-fold. Results: ZG16p binds to short α-mannose-related glycans and glycosaminoglycans via the canonical shallow mannose-binding pocket and an adjacent basic surface area, respectively. Conclusion: ZG16p possesses a unique feature of multiple-ligand binding. Significance: Structural insights of ZG16p ligand binding are crucial for understanding the biological functions of the protein. Article type: Research Article Keywords: Crystal Structure, Heparin, Lectin, Microarray, Nuclear Magnetic Resonance (NMR), ZG16p, Carbohydrate Recognition, Mannose Authors: Mayumi