The structure of Rv3717 reveals a novel amidase from Mycobacterium tuberculosis

Abstract The structure of Rv3717 determined to 1.7 Å resolution by Pt-SAD phasing reveals a unique autolysin that lacks a cell-wall-binding domain. Rv3717 utilizes its net positive charge for substrate binding and exhibits activity towards a broad spectrum of substrate cell walls. Structural analysis reveals that Rv3717 utilizes a β-hairpin turn at its N-terminus to autoregulate its enzymatic activity. Article type: Research Article Keywords: Rv3717, SAD phasing, LC/MS analysis, α/β-fold Authors: Atul Kumar, Sanjiv Kumar, Dilip Kumar, Arpit Mishra, Rikeshwer