Comprehensive analysis of BIRC5: from pan-cancer analysis to experimental validation

Abstract Background: Baculoviral inhibitor of apoptosis protein repeat-containing protein 5 (BIRC5), also known as survivin, belongs to the inhibitor of apoptosis proteins (IAP) family and serves as a key regulator of cellular survival and programmed cell death. Current research has demonstrated that BIRC5 exhibits high expression levels in many cancers. Furthermore, its overexpression significantly correlates with poor clinical outcomes and contributes to tumor progression and metastasis. Methods: We utilized TCGAplot R package (v8.0.0), Sangerbox3.0, GEPIA2, cBioPortal, UALCAN, TISIDB, BioGRID, and