Study on the Screening of Anti‐Osteoporosis Components in Processed Epimedium‐Based on Spectrum‐Effect Analysis

Abstract Epimedium (EP) is a traditional Chinese medicine that has been used to treat osteoporosis for several years, but its mechanism of action is unclear. We used ultrahigh‐performance liquid chromatography coupled with triple–quadrupole mass spectrometry (UPLC–QQQ–MS/MS) to detect the components of raw and processed EP samples. The antioxidant activities of the various tested compounds were evaluated using 1,1‐diphenyl‐2‐trinitrophenylhydrazine (DPPH), and their antiosteoporosis effects were assessed in H2O2‐stimulated MC3T3 cells and zebrafish embryos. The correlation between the chemical ingredients and efficacy