Multi-omics analysis of tumor necrosis factor superfamily 4 reveals its prognostic value with T cell exhaustion feature in cancer

Abstract Background: As a member of the tumor necrosis factor (TNF) family, TNF superfamily 4 (TNFSF4) plays a crucial role in various immune-related processes. However, its biological function in pan-cancer remains largely unexplored. Methods: RNA-sequencing (RNA-seq) data and corresponding clinical variables were obtained from the Cancer Genome Atlas (TCGA). Immunotherapy cohorts were retrieved from Gene Expression Omnibus (GEO). Tumor Immune Estimation Resource was used to evaluate tumor-infiltrating immune cell levels. The Tumor Immune Single-cell Hub (TISCH) 2 database was used