LC–MS/MS Profiling, Biological Activities and Molecular Docking Studies of Simmondsia Chinensis Leaves

Abstract This study aimed to assess the biological activities of Tunisian Simmondsia chinensis and characterize its potential bioactive compounds. Different extracts of S. chinensis were tested for their antioxidant, antibacterial, anti‐α‐amylase, and anti‐acetylcholinesterase activities through in vitro assays. The methanolic extract exhibited the highest levels of antioxidant activity and total phenolic content (976.03 GAE/g extract) compared to the other extracts. Additionally, it demonstrated a substantial anti‐acetylcholinesterase activity (PI=75 %) and potent antibacterial property, particularly against Enterococcus faecalis, Listeria monocytogenes, Bacillus