Unveiling the Substrate‐Dependent Dynamics of Mycotoxin Production in Fusarium verticillioides Using an OSMAC‐Metabolomics Approach

Abstract Fusarium verticillioides is a prevalent plant pathogenic fungus known to produce harmful mycotoxins, including fumonisins and emerging toxins. This study aimed to investigate the influence of substrate on the temporal patterns of mycotoxin biosynthesis by F. verticillioides, employing a combined OSMAC (One Strain‐Many Compounds) strategy and metabolomics approach. The fungus was cultured under various media conditions, and samples were collected over time. LC–MS/MS analyses and a dereplicative workflow were used to profile the secondary metabolite production, focusing on mycotoxins.