Discovery of major QTL and a massive haplotype associated with cannabinoid biosynthesis in drug‐type Cannabis

Abstract Cannabis (Cannabis sativa L.), once sidelined by decades of prohibition, has now gained recognition as a multifaceted and promising plant in both medical research and commercial applications following its recent legalization. This study leverages a genome‐wide association study (GWAS) on 174 drug‐type Cannabis accessions from the legal Canadian market, focusing on identifying quantitative trait loci (QTL) and candidate genes associated with eleven cannabinoid traits using 282K common single‐nucleotide polymorphisms. This approach aims to transform our understanding of Cannabis genetics.