Comparing the Utility of Cannabidiol Quantitation Methods for Use in High-Throughput In Vitro Assays

Abstract In recent years, isolated phytocannabinoids have received significant attention for their therapeutic potential, showing diverse pharmacological effects in vitro and in preclinical studies. However, in vitro drug efficacy and drug development studies are challenging to perform as accurate quantification of phytocannabinoids is required to generate dose response curves and pharmacokinetic profiles. While assessment of free cannabinoids is straightforward, assessing small quantities in cell culture media or as part of a formulation is difficult due to the low solubility of