Steroid Hormone Changes after Short-Term High-Dose Exposure to Cannabidiol in Healthy Subjects: An Exploratory Study

Abstract Introduction: Cannabis-derived cannabidiol (CBD; Epidiolex®) is FDA-approved for seizures associated with Lennox-Gastaut syndrome, Dravet syndrome, and tuberous sclerosis complex, yet its effects on human steroid hormone regulation remain unclear. This study evaluated changes in five plasma steroid hormones and one precursor bile acid after short-term high-dose oral CBD administration in healthy subjects. Methods: Twelve participants (6 males, 6 females) completed a randomized, placebo-controlled crossover study and received oral CBD 750 mg twice daily for 3.5 days or placebo, separated