Multiplex high resolution melting using MADC2 and SCAR markers for sex identification of Cannabis sativa L. cultivation

Abstract Supplementary Information: The online version contains supplementary material available at 10.1038/s41598-026-46461-6. Article type: Research Article Keywords: adulteration, DNA fingerprint, melting temperature, molecular markers, multiplex detection, Genotyping and haplotyping, Plant breeding Authors: Maturada Siengthong, Puntitra Kamol, Apitssamai Yodkham, Phuping Sucharitakul, Siriwadee Chomdej, Kawee Sujipuli, Kittisak Buddhachat Affiliations: https://ror.org/03e2qe334grid.412029.c0000 0000 9211 2704Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, 65000 Thailand; https://ror.org/00a5mh069grid.412996.10000 0004 0625 2209Biology Program, School of Science, University of Phayao, Phayao, 56000 Thailand; https://ror.org/03cve4549grid.12527.330000 0001 0662 3178School of