Comparing methods for controlled capture and quantification of pollen in Cannabis sativa

Abstract Premise: Precise pollen collection methods are necessary for crop breeding, but anemophilous pollen is notoriously difficult to capture and control. Here we compared a variety of methods for the controlled capture of cannabis pollen, intended to ease the process of cross‐fertilization for breeding this wind‐pollinated plant, and measured the utility of light spectroscopy for quantifying relative pollen yield. Methods and Results: In two independent trials, we compared a control method of pollen collection (hand collection) to either vacuum‐, water‐,