A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L
Abstract Background: Protoplasts are a valuable tool for studying gene expression and applying genome editing techniques. Given the high medicinal…
Andrew Maxwell Phineas Jones
Decoding the cannabis tissue culture puzzle: Machine learning analysis of cannabis in vitro morpho-physiological disorders expands the potential for precision micropropagation
Abstract Machine learning was applied to detect nutrient-related in vitro cannabis symptoms. Media formulations were evaluated for impact on morpho-physiological…
Bottom Cooling During Culture Initiation Increases Survival and Reduces Hyperhydricity in Micropropagated Cannabis Plants
Abstract Hyperhydricity is characterized by morphological abnormalities and reduced plant vigour. This study investigated the use of a bottom cooling…
Machine Learning-Aided Optimization of In Vitro Tetraploid Induction in Cannabis
Abstract Polyploidy, characterized by an increase in the number of whole sets of chromosomes in an organism, offers a promising…
Somatic Mutation Accumulations in Micropropagated Cannabis Are Proportional to the Number of Subcultures
Abstract Advancements in micropropagation techniques have made it easier to produce large numbers of cannabis clones, but these methods may…
Comparative restriction enzyme analysis of methylation (CREAM) reveals methylome variability within a clonal in vitro cannabis population
Abstract The primary focus of medicinal cannabis research is to ensure the stability of cannabis lines for consistent administration of…
Naturally Occurring Triploidy in Cannabis
Abstract Polyploidy is a significant evolutionary process in plants that involves the duplication of genomic content and has been recognized…
Morphological Characterization of Cannabis sativa L. Throughout Its Complete Life Cycle
Abstract This study extensively characterizes the morphological characteristics, including the leaf morphology, plant structure, flower development, and trichome features throughout…
Transcriptomic Profiling of Embryogenic and Non-Embryogenic Callus Provides New Insight into the Nature of Recalcitrance in Cannabis
Abstract Differential gene expression profiles of various cannabis calli including non-embryogenic and embryogenic (i.e., rooty and embryonic callus) were examined…
Machine Learning-Mediated Development and Optimization of Disinfection Protocol and Scarification Method for Improved In Vitro Germination of Cannabis Seeds
Abstract In vitro seed germination is a useful tool for developing a variety of biotechnologies, but cannabis has presented some…