Development and Characterization of Cannabidiol Gummy Using 3D Printing
Abstract Oropharyngeal dysphagia and pain are prevalent concerns in the geriatric population. Therefore, this study investigates advances in the development…
Research Article
CB1 cannabinoid receptor agonists induce acute respiratory depression in awake mice☆
Abstract Recreational use of synthetic cannabinoid agonists (i.e., “spice compounds”) that target the cannabinoid type 1 receptor (CB1) can cause…
超高效液相色谱-三重四极杆质谱法测定大口黑鲈和草鱼胆汁中30种胆汁酸
Abstract 胆汁酸(bile acids, BAs)是胆汁的主要成分,在糖脂和胆固醇的代谢中具有重要的意义。大口黑鲈(Micropterus salmoides)和草鱼(Ctenopharyngodon idella)都是我国重要的淡水鱼养殖品种,明确两种鱼胆汁中胆汁酸谱可为该养殖领域提供数据支持。本研究利用超高效液相色谱-三重四极杆质谱开发了一种能同时检测30种胆汁酸的定性定量方法,并应用于大口黑鲈和草鱼胆汁中胆汁酸的种类和含量分析。方法优化了样品处理方法和仪器分析条件。胆汁样本经过离心后吸取上清液,以甲醇为提取溶剂涡旋混匀,经0.22 μm滤膜过滤后上机分析。采用Shim-pack Velox SP-C18色谱柱(100 mm×2.1 mm, 1.8 μm)进行分离,以0.01%冰乙酸水溶液-乙腈为流动相进行梯度洗脱,柱温50 ℃,进样量2 μL,采用电喷雾离子源(ESI)进行8种正离子、22种负离子的多反应监测(MRM)扫描。结果表明,30种胆汁酸在各自线性范围内线性良好,相关系数(R2)为0.9975~0.9997,回收率为72.3%~117.2%,相对标准偏差为0.46%~13.23%,方法的检出限(LOD)为0.01~0.75 ng/mL,定量限(LOQ)为0.02~2.28 ng/mL。利用所建立的方法对大口黑鲈和草鱼的胆汁进行检测,在大口黑鲈胆汁中成功检测出19种胆汁酸;在草鱼胆汁中成功检测出16种胆汁酸,大口黑鲈胆汁中有5种胆汁酸在草鱼胆汁中未检出,而草鱼胆汁中有2种胆汁酸在大口黑鲈胆汁中未检出。该方法简单快速,灵敏度和精确度高,适用于大口黑鲈和草鱼胆汁中30种胆汁酸的同时检测。 Article type: Research…
Transcriptomic changes in oxidative stress, immunity, and cancer pathways caused by cannabis vapor on alveolar epithelial cells
Abstract Graphical Abstract: • Vaporizing cannabis is increasingly popular but remains largely untested. • We used three in vitro models…
A Survey on the Use of Cannabidiol (CBD) Isolate, Its Perceived Benefits, and Associated Side Effects Among Subjects With Chronic Pain
Abstract Introduction: Chronic pain is a pervasive health issue in the United States, affecting up to 50 million adults and…
Pilot Study on the Effect of Cannabidiol-Coated Fabric for Pillow Covers Improves the Sleep Quality of Shift Nurses
Abstract Background: Sleep difficulty is common in the current society. Poor sleep has a significant influence on health, social interactions…
Comprehensive analysis of TMEM9 in human tumors
Abstract Background: TMEM9, a transmembrane protein, has emerged as a significant player in tumor progression, yet its comprehensive role across…
Pan‑cancer analysis of oncogene SFXN1 to identify its prognostic and immunological roles in lung adenocarcinoma
Abstract As cancer incidence and mortality rates continue to rise, the urgency for research in this field has increased globally.…
FN1 Immunoregulation in Glioblastoma: Insights From Neutrophil‐Centric Studies
Abstract Neutrophils, as key components of the tumor microenvironment, play a crucial role in cancer progression and prognosis. This study…
Diagnostic, prognostic, and immunological roles of NCAPG in pan-cancer: A bioinformatics analysis
Abstract Growing studies have shown that non-SMC condensin I complex subunit G (NCAPG) was highly expressed in a variety of…