TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells
Abstract Recognition of specific chromatin modifications by distinct structural domains within “reader” proteins plays a critical role in the maintenance…
Structural biology
Histone chaperone exploits intrinsic disorder to switch acetylation specificity
Abstract Histones, the principal protein components of chromatin, contain long disordered sequences, which are extensively post-translationally modified. Although histone chaperones…
Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering
Abstract Myosin VI plays crucial roles in diverse cellular processes. In autophagy, Myosin VI can facilitate the maturation of autophagosomes…
Tandem hnRNP A1 RNA recognition motifs act in concert to repress the splicing of survival motor neuron exon 7
Abstract HnRNP A1 regulates many alternative splicing events by the recognition of splicing silencer elements. Here, we provide the solution…
The Ki-67 and RepoMan mitotic phosphatases assemble via an identical, yet novel mechanism
Abstract Ki-67 and RepoMan have key roles during mitotic exit. Previously, we showed that Ki-67 organizes the mitotic chromosome periphery…
Studying the Structure and Dynamics of Biomolecules by Using Soluble Paramagnetic Probes
Abstract Characterisation of the structure and dynamics of large biomolecules and biomolecular complexes by NMR spectroscopy is hampered by increasing…
Selective Recruitment of an E2∼Ubiquitin Complex by an E3 Ubiquitin Ligase*
Abstract Background: Rbx1/ROC1 is an E3 ligase adaptor protein that functions with the E2 enzyme CDC34. Results: NMR and biochemical…