Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins
Abstract Background: Some peptides are targets for degradation when heterologously expressed as fusion proteins in E. coli, which can limit…
Protein purification
Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.)
Abstract Sucrose synthase is a key enzyme in sucrose metabolism as it saves an important part of sucrose energy in…