Trans-ancestry epigenome-wide association meta-analysis of DNA methylation with lifetime cannabis use
Abstract Cannabis is widely used worldwide, yet its links to health outcomes are not fully understood. DNA methylation can serve…
Molecular biology
PPARα and PPARγ are expressed in midbrain dopamine neurons and modulate dopamine- and cannabinoid-mediated behavior in mice
Abstract Peroxisome proliferator-activated receptors (PPARs) are a family of nuclear receptors that regulate gene expression. Δ9-tetrahydrocannabinol (Δ9-THC) is a PPARγ…
Alpha-linolenic acid modulates systemic and adipose tissue-specific insulin sensitivity, inflammation, and the endocannabinoid system in dairy cows
Abstract Metabolic disorders are often linked to alterations in insulin signaling. Omega-3 (n-3) fatty acids modulate immunometabolic responses; thus, we…
Chronic low-dose Δ9-tetrahydrocannabinol (THC) treatment stabilizes dendritic spines in 18-month-old mice
Abstract Cognitive functions decline during aging. This decline could be caused by changes in dendritic spine stability and altered spine…
Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy
Abstract Dusquetide is a next-generation IDR (innate defense regulator) targeting the major autophagy receptor protein SQSTM1/p62 and modulating the innate…
Cannabidiol affects breast meat volatile compounds in chickens subjected to different infection models
Abstract No study has demonstrated the use of dietary Cannabis-derived cannabidiol (CBD) to alter the stress response in chickens or…
New AKT-dependent mechanisms of anti-COVID-19 action of high-CBD Cannabis sativa extracts
Abstract COVID-19 is caused by the SARS-CoV-2 virus, which enters target cells via interactions with ACE2 and TMPRSS2. Here, we…
Biomarkers of the Endocannabinoid System in Substance Use Disorders
Abstract Despite substance use disorders (SUD) being one of the leading causes of disability and mortality globally, available therapeutic approaches…
A fluorescence recovery after photobleaching protocol to measure surface diffusion of DAGLα in primary cultured cortical mouse neurons
Abstract This protocol describes using fluorescence recovery after photobleaching (FRAP) of a superecliptic pHluorin (SEP)-diacylglycerol lipase α (DAGLα) to measure…
UV induced changes in proteome of rats plasma are reversed by dermally applied cannabidiol
Abstract UV radiation is known to induce a multiple changes in the metabolism of skin-building cells, what can affect the…