Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins
Abstract Background: Some peptides are targets for degradation when heterologously expressed as fusion proteins in E. coli, which can limit…
intein
Study on Cecropin B2 Production via Construct Bearing Intein Oligopeptide Cleavage Variants
Abstract In this study, genetic engineering was applied to the overexpression of the antimicrobial peptide (AMP) cecropin B2 (cecB2). pTWIN1…
Optimization and One-Step Purification of Recombinant V Antigen Production from Yersinia pestis
Abstract The purpose of this study was to develop an efficient and inexpensive method for the useful production of recombinant…