Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins
Abstract Background: Some peptides are targets for degradation when heterologously expressed as fusion proteins in E. coli, which can limit…
Fusion proteins
NMR structure and localization of a large fragment of the SARS-CoV fusion protein: Implications in viral cell fusion
Abstract The lethal Coronaviruses (CoVs), Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) and most recently Middle East Respiratory Syndrome Coronavirus, (MERS-CoV)…
A new fusion protein platform for quantitatively measuring activity of multiple proteases
Abstract Background: Recombinant proteins fused with specific cleavage sequences are widely used as substrate for quantitatively analyzing the activity of…
Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum β-glucosidase fused to Clostridium cellulovorans cellulose binding domain and its application in hydrolysis of microcrystalline cellulose
Abstract Background: The complete degradation of the cellulose requires the synergistic action of endo-β-glucanase, exo-β-glucanase, and β-glucosidase. But endo-β-glucanase and…
Expression, Immobilization and Enzymatic Properties of Glutamate Decarboxylase Fused to a Cellulose-Binding Domain
Abstract Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion of glutamic acid to gamma-aminobutyric acid (GABA), was…