X-ray crystal structures of the cannabinoid synthases CBCAS, CBDAS and THCAS
Abstract The enzymes Cannabichromenic Acid Synthase (CBCAS), Cannabidiolic Acid Synthase (CBDAS) and Tetrahydrocannabinolic Acid Synthase (THCAS) are together the major…
biocatalysis
Engineering a promiscuous prenyltransferase for selective biosynthesis of an undescribed bioactive cannabinoid analog
Abstract Cannabinoids are unique meroterpenoids, with cannabigerolic acid (CBGA) serving as a dedicated precursor. This study introduces a fungal aromatic…
The evolutionary origin of naturally occurring intermolecular Diels-Alderases from Morus alba
Abstract Biosynthetic enzymes evolutionarily gain novel functions, thereby expanding the structural diversity of natural products to the benefit of host…
A cell-free platform for the prenylation of natural products and application to cannabinoid production
Abstract Prenylation of natural compounds adds structural diversity, alters biological activity, and enhances therapeutic potential. Because prenylated compounds often have…