Abstract Background: Dietary intervention-induced modulation of bile acids may enhance metabolic homeostasis and facilitate weight reduction. We aimed to elucidate…
Abstract Dietary fibre can modify colostrum and milk composition in sows. Bile acids (BA) aid in fat digestion and lipid…
Abstract Background: Circulating bile acids (cBAs) function as signaling molecules that activate the farnesoid X receptor (FXR), promoting the secretion…
Abstract Background: Clostridioides difficile infection (CDI) is the leading cause of nosocomial diarrhea. Current diagnostic tools have difficulty distinguishing between…
Abstract Background: Type 2 diabetes mellitus (T2DM), characterized by impaired glucose homeostasis, represents a significant threat to public health. Bile…
Abstract Background: During the transition period, cows are prone to negative energy balance, which can lead to a decline in…
Abstract 胆汁酸(bile acids, BAs)是胆汁的主要成分,在糖脂和胆固醇的代谢中具有重要的意义。大口黑鲈(Micropterus salmoides)和草鱼(Ctenopharyngodon idella)都是我国重要的淡水鱼养殖品种,明确两种鱼胆汁中胆汁酸谱可为该养殖领域提供数据支持。本研究利用超高效液相色谱-三重四极杆质谱开发了一种能同时检测30种胆汁酸的定性定量方法,并应用于大口黑鲈和草鱼胆汁中胆汁酸的种类和含量分析。方法优化了样品处理方法和仪器分析条件。胆汁样本经过离心后吸取上清液,以甲醇为提取溶剂涡旋混匀,经0.22 μm滤膜过滤后上机分析。采用Shim-pack Velox SP-C18色谱柱(100 mm×2.1 mm, 1.8 μm)进行分离,以0.01%冰乙酸水溶液-乙腈为流动相进行梯度洗脱,柱温50 ℃,进样量2 μL,采用电喷雾离子源(ESI)进行8种正离子、22种负离子的多反应监测(MRM)扫描。结果表明,30种胆汁酸在各自线性范围内线性良好,相关系数(R2)为0.9975~0.9997,回收率为72.3%~117.2%,相对标准偏差为0.46%~13.23%,方法的检出限(LOD)为0.01~0.75 ng/mL,定量限(LOQ)为0.02~2.28 ng/mL。利用所建立的方法对大口黑鲈和草鱼的胆汁进行检测,在大口黑鲈胆汁中成功检测出19种胆汁酸;在草鱼胆汁中成功检测出16种胆汁酸,大口黑鲈胆汁中有5种胆汁酸在草鱼胆汁中未检出,而草鱼胆汁中有2种胆汁酸在大口黑鲈胆汁中未检出。该方法简单快速,灵敏度和精确度高,适用于大口黑鲈和草鱼胆汁中30种胆汁酸的同时检测。 Article type: Research…
Abstract The aim of this study was to investigate the protective effects and potential mechanisms of Tetrahydrocurcumin (THC) on methionine–choline-deficient…
Abstract Maternal inulin intake has been shown to alleviate oxidative stress in piglets, but the role of bile acids (BAs)…
Abstract Stable-isotope dilution MS/MS assay determines all 8 atypical C27 bile acids. C27 bile acids accumulate in patients with peroxisomal…