Structural basis for activation of CB1 by an endocannabinoid analog
Abstract Endocannabinoids (eCBs) are endogenous ligands of the cannabinoid receptor 1 (CB1), a G protein-coupled receptor that regulates a number…
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Assessing the impact of piercing-sucking pests on greenhouse-grown industrial hemp (Cannabis sativa L.)
Abstract Cannabis sativa or hemp, defined as <0.3% total tetrahydrocannabinol (THC), is a specialty crop in the United States, of…
Treatment of Posttraumatic Stress Disorder: A State-of-the-art Review
Abstract This narrative state-of-the-art review paper describes the progress in the understanding and treatment of Posttraumatic Stress Disorder (PTSD). Over…
增强型脂质去除净化剂结合超高效液相色谱-串联质谱法测定食品中8种大麻素类化合物
Abstract 研究在优化前处理条件及色谱分离的基础上,建立了同时测定巧克力、软糖、糕点、饼干、饮料、白酒等6种典型食品基质中8种大麻素类化合物的超高效液相色谱-串联质谱(UPLC-MS/MS)的测定方法。前处理方法中考察了不同的净化方式,采用增强型脂质去除净化剂(EMR-Lipid)解决了巧克力中复杂油脂及软糖中胶质难以去除的问题;同时净化溶液在氮吹前加入10%丙三醇甲醇溶液,解决了目前文献方法中直接氮吹对回收率影响很大的关键问题。样品以乙腈为提取溶剂,EMR-Lipid为净化剂进行净化,无水硫酸钠除水后,乙腈层加入10%丙三醇甲醇溶液100 μL,氮吹至近干;采用Waters ACQUITY UPLC BEH Shield RP18色谱柱进行分离,电喷雾负离子扫描,多反应监测(MRM)模式检测,基质匹配外标法定量。方法学研究表明,四氢大麻酚、次大麻二酚、四氢大麻素、大麻萜酚在2~200 ng/mL范围内线性关系良好;大麻酚、大麻二酚、大麻二酚酸、四氢大麻酚酸A在0.4~40 ng/mL范围内线性关系良好,相关系数(r)均大于0.995;检出限和定量限分别为0.8~4 μg/kg和2~10 μg/kg。在不同加标水平下的平均回收率为82.0%~114.9%,相对标准偏差(RSD)均小于15%(n=6)。研究结果表明,采用EMR-Lipid净化剂净化食品基质时,净化效果明显,准确度较好。该方法灵敏、快速,准确度高,前处理简单,适用于食品基质中大麻素类化合物的准确定性定量检测。 Article type: Research Article Keywords: 高效液相色谱-串联质谱, 增强型脂质去除净化剂, 大麻素类化合物,…
Treating nightmares in posttraumatic stress disorder with dronabinol: study protocol of a multicenter randomized controlled study (THC PTSD-trial)
Abstract Background: Distressing nightmares are a core symptom of posttraumatic stress disorder (PTSD) and contribute to psychiatric comorbidity, impaired physical…
Substance abuse and susceptibility to false memory formation: a systematic review and meta-analysis
Abstract Background: Substance abuse has an impact on various cognitive domains, including memory. Even though this impact has been extensively…
The Effects of a Combination of Medical Cannabis, Melatonin, and Oxygen–Ozone Therapy on Glioblastoma Multiforme: A Case Report
Abstract Glioblastoma is the most aggressive malignant tumor overall and remains an incurable neoplasm with a median survival of 15…
Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
Abstract Human immunodeficiency virus (HIV) infection is associated with a chronic inflammatory stage and continuous activation of inflammasome pathway. We…
Effects of Cannabidiol, Hypothermia, and Their Combination in Newborn Rats with Hypoxic-Ischemic Encephalopathy
Abstract Therapeutic hypothermia is well established as a standard treatment for infants with hypoxic-ischemic (HI) encephalopathy but it is only…
Transcriptomic signature, bioactivity and safety of a non-hepatoxic analgesic generating AM404 in the mid-brain PAG region
Abstract The safe and effective management of pain is a critical healthcare and societal need. The potential for misuse and…