Biomimetic Partitioning and Pharmacokinetic Insights into Phytocannabinoids

Abstract Phytocannabinoids from Cannabis sativa exhibit therapeutic potential, yet historical research restrictions have limited the ability to acquire key physicochemical data. This study employs biomimetic chromatography to characterize the partitioning behavior of eight neutral and seven acidic phytocannabinoids using octadecylsilica (C18), an immobilized artificial membrane (IAM), human serum albumin (HSA), and α-1-acid glycoprotein (AGP) columns. Chromatographic hydrophobic index (CHI) scaled to octanol–water partition coefficient (log D) values ranged from 4.16 to 6.06 for neutral compounds and 2.09–3.15 for acidic ones.