Acanthospermum hispidum and Its Metabolite Stigmasterol Modulates Anti‐Inflammatory Antagonism of COX‐1 and TGF‐β

Abstract Acanthospermum hispidum (AH) leaves have been widely used in traditional medicine for inflammation. In this study, the anti‐inflammatory effect of AH was evaluated, and a possible mechanism was predicted using an in silico model. The in vivo anti‐inflammatory activity of the AH extract was carried out using carrageenan, histamine and serotonin‐induced rat paw oedema, whereas molecular docking was used against all receptors implicated with inflammation. AH extract showed significant (p < 0.01) inhibition at 50, 100, 150 and 200